oriTDB

The information of the oriT region

oriTDB accession number	100847
Name	oriT_pSA4
Sequence Completeness	intact
oriT length	191 nt
oriT sequence	GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA CATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAG CGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGAC GGTAAAAGCTA
IR (inverted repeat)	IR1: [19-38] [39-58] nt (AACCGTGAAGCGCGATCCGA..TAGGATTGAGCGTATCGGTT) IR2: [119-128] [131-140] nt (AGCGGTGGCA..TGCCACCGCT)
Location of nic site	[86-87] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:19-38;R:39-58;L:119-128;R:131-140;D:86-87

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA

61

CATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAG

121

CGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGAC

181

GGTAAAAGCTA

Reference
N/A

I. Information of Relaxase

ID	919
Name	TraA_pSA4
GenBank accession number	WP_007686163
Family	MOB_Q
Length	1043 aa
UniProt ID	A0A1D8AGX6
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.50E-70, Aligned region: 17..217 AAA_30 [PF13604.5], Evalue: 3.40E-58, Aligned region: 346..535 AAA_19 [PF13245.5], Evalue: 6.50E-08, Aligned region: 351..478
Note	putative relaxase
Protein sequence [Download]	MAIYHFSAKVISRANGSSAVASAAYRAAERLHDDRLGRDHDFSNKAGVVHSEIMLPEGTP ERLNDRATLWNEVEAGEKRKDAQLAREVEFSIPRELDQKEGIRLARDFVEKQFVERGMVA DMNVHWDLGKDGQSKPHAHVMLSMREVGLEGFGQKVREWNSTALLQEWREAWADHVNERL AELDIDARIDHRSYEAQGIELEPQHKIGPAASRMPEQGLEAERVEDHARIARENGEKIIA NPQIALDAITRQQATFTRRDLAQFAFRHSDGKDQFDQVMSAVRTSPELVALGKDGRGEDR FTSREMIDTEQRLERAGYGLADRAGHGLPAASKMGGRDTAERVGLALGSQQKDALAHITG KNDLAIVVGYAGTGKSTMLGVARDEWERAGYQVRGAALSGIAAEGLEGGSGIASRTIASM EYQWEQGRELLGPRDVLVIDEAGMIGTRQMERVLSEAERAGAKVILVGDAEQLQAIEAGA AFRSLAERYGAAEISDVRRQHEDWQKDATRALATGRTGEAIHAYAEHGMVHAAETREAAR ESLVEGWDRARIADPDKTRIILTHTNAEVRDLNLAARDRLRDAGELGQDVRVSAERGARE FATGDRIMFLKNDRGLGVKNGTLGKVERVSPDSMAVKLDDGRQVAFDLKDYAHVDHGYAA TIHKSQGVTVDRAHVLATPGMDRHSAYVALSRHRDGVQLHYGRDDFADDRRLVRTLSRER AKDMASDYPMYRDRDAEAQSFADRRGLSGEIRVADAPERKGVEILAPRAGTSRQMGEDSR TPDIGGDRGAGEAKAAAERQSRRGMFDGLKLSAAPVKAAEPAQGAKEKPAPKRGMFDGLK LPQRTPTPEPAKELPARAEQGQDRDFRRAVERASRSAEAVLQARASGGPVLEHQKVALER AVQALDTLRPRASDDMAEAMKRDSGLLREAAAGRSGPMIEAIRQEARVRADPNLRADRFV ERWQQLSQDRDRLYRAGDMAGRKTLGQEMAGMAKSLERDPQVESILRGRTRELGLEIGMS PGRELGRELSRGLGISHDRGLSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1307
Plasmid name	pSA4
GenBank accession number	NZ_CP017079.1
Genome size	67284 bp
Coordinate of oriT [Strand]	59882..60072 [-]
Drug resistance	resistance to hydrophobic antibiotics such as nalidixic acid
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Novosphingobium resinovorum SA1 [158500]

Reference
N/A