oriTDB
The information of the oriT region
oriTDB accession number 100844
Name oriT_pNCI002  insolico
Sequence Completeness core
oriT length 34 nt
oriT sequence GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)[4-18] [22-34] nt  (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site [2-3]nt
Conserved sequence flanking the
  nic site 		_
Note predicted by the oriTfinder
L:4-18;R:22-34;D:2-3
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 916
Name Nickase_pNCI002 insolico
GenBank accession number WP_042385886
Family MOBQ
Length 643 aa
UniProt ID _
PDB ID _
Pfam MobA_MobL [PF03389.14], Evalue: 2.20E-71, Aligned region: 16..223
Note putative relaxase
Protein sequence [Download] MAYFLFRTQIISKSNRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPE
WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD
IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD
RINEKFKEKGIDDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY
YGKLNQEIQRINQELKALKQKEKVVSISDYQEEKSLNETIESIRKNASLNDVEKASLLLV
AKRAKTYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPKQVIQY
GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH
AGADFRIEEKYHAVQYFKDTGGVLPESDIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL
NRTIQKQSKERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG
RDVSITNTAALLRLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR
QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1304
Plasmid name pNCI002
GenBank accession number NZ_CP016918.1
Genome size 47892 bp
Coordinate of oriT  [Strand] 12520..12553 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Parageobacillus thermoglucosidans TM242 [1426]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU