oriTDB
The information of the oriT region
oriTDB accession number 100843
Name oriT_pJM1B  insolico
Sequence Completeness intact
oriT length 460 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCATAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTAATAGCTTGCCAGTATTTA TGGTTTTATATGGTCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGA AGTTCTTGATGGATCAGAAAAATGTATCACAAATTTAAAC
IR (inverted repeat)IR1: [138-147] [152-161] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [207-219] [223-235] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-255] [268-281] nt  (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site [290-291] nt
Conserved sequence flanking the
  nic site 		CTTG|CC
Note predicted by the oriTfinder
L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D:290-291
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 915
Name PriA_pJM1B insolico
GenBank accession number WP_050595623
Family MOBP
Length 380 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.20E-20, Aligned region: 1..137
Note putative relaxase
Protein sequence [Download] MVLNATNPLTLNKFQQNKNDLERLKEISDNISKEYGCKIIVRPEQKLGNSHKNYLVYLAK
NSYRKEIKNKLDFLMNHSHTWEDFKEKARALNLKVDDTKKYTTYLLEGSEQTKKIRDRSL
KNDKFLKENLKERIEKNTIGYSVDEVVKLWKDKESIQEKGREKEIEILLEHWQVTKETEK
DLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYLDKKSPANHKIMYGATV
AKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLSFDRSFETIGEELKATYQETQHQ
LDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQKSEQRL
EAYQKNQSPKHKARDDDFEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1303
Plasmid name pJM1B
GenBank accession number NZ_CP016748.1
Genome size 48280 bp
Coordinate of oriT  [Strand] 37631..38090 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. cremoris JM1 [1359]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU