oriTDB
The information of the oriT region
oriTDB accession number 100842
Name oriT_pJM3C  insolico
Sequence Completeness intact
oriT length 482 nt
oriT sequence CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATT GTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTT ATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAAT CAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTT GATGGATCAGAAAAATGTATCACAAATTTAAACAAAGACTCACTCATTTAAGAGACGCTA CTAGCATGAAATTTTGTTGTTGCGATAAGCAACTTCTGATACACGATTTTTAGCCATTAC ATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGC CGACCAGCGTTTGAAACTTTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTC GA
IR (inverted repeat)IR1: [21-33] [37-49] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR2: [55-69] [82-96] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [104-105] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L: 21-33;R:37-49;L:55-69;R:82-96;D:104-105
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 914
Name Relaxase_pJM3C insolico
GenBank accession number WP_063284081
Family MOBP
Length 410 aa
UniProt ID A0A1V0P6Q0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 9.40E-75, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK
DKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1302
Plasmid name pJM3C
GenBank accession number NZ_CP016739.1
Genome size 45257 bp
Coordinate of oriT  [Strand] 13398..13879 [-] [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. cremoris JM3 [1359]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU