oriTDB

The information of the oriT region

oriTDB accession number	100842
Name	oriT_pJM3C
Sequence Completeness	intact
oriT length	482 nt
oriT sequence	CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATT GTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTT ATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAAT CAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTT GATGGATCAGAAAAATGTATCACAAATTTAAACAAAGACTCACTCATTTAAGAGACGCTA CTAGCATGAAATTTTGTTGTTGCGATAAGCAACTTCTGATACACGATTTTTAGCCATTAC ATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGC CGACCAGCGTTTGAAACTTTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTC GA
IR (inverted repeat)	IR1: [21-33] [37-49] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR2: [55-69] [82-96] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[104-105] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L: 21-33;R:37-49;L:55-69;R:82-96;D:104-105

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGACACACTCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCCACATT

61

GTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATGGGTTT

121

ATATTTCCTATTTTGTTGTGAGGATTGTAACCGAATAGGGCGCAATACTTATTACAAAAT

181

CAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGGAAGTTCTT

241

GATGGATCAGAAAAATGTATCACAAATTTAAACAAAGACTCACTCATTTAAGAGACGCTA

301

CTAGCATGAAATTTTGTTGTTGCGATAAGCAACTTCTGATACACGATTTTTAGCCATTAC

361

ATCACTCGTTTTTAGAGTGATGTGTAAGTGCGCATTGCACTCTTTTTTAACGAAACAAGC

421

CGACCAGCGTTTGAAACTTTTTAGTTTTTCATCATTCTATTTTAAAACGCTCTAAAACTC

481

GA

Reference
N/A

I. Information of Relaxase

ID	914
Name	Relaxase_pJM3C
GenBank accession number	WP_063284081
Family	MOB_P
Length	410 aa
UniProt ID	A0A1V0P6Q0
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.40E-75, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVIRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDVSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESTINRFIDTIKQFAGRVPELTQRVTRKLKQTK DKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1302
Plasmid name	pJM3C
GenBank accession number	NZ_CP016739.1
Genome size	45257 bp
Coordinate of oriT [Strand]	13398..13879 [-] [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. cremoris JM3 [1359]

Reference

[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]