 oriTDB accession number | 100841 |
| Name | oriT_pJM3A  |
Sequence Completeness  | intact |
| oriT length | 463 nt |
| oriT sequence | TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT AGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAA GTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG |
| IR (inverted repeat) | IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-255] [268-281] nt (ACATTGTAATACAA..TTGTATTACAATGT) |
| Location of nic site | [290-291] nt |
| Conserved sequence flanking the nic site | CTTG|CA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 913 |
| Name | Relaxase_pJM3A |
| GenBank accession number | WP_011669060 |
| Family | MOBP |
| Length | 135 aa |
| UniProt ID | A0A1V0P5P3 |
| PDB ID | _ |
| Pfam | Relaxase [PF03432.13], Evalue: 1.40E-20, Aligned region: 16..124 |
| Note | putative relaxase |
| Protein sequence [Download] | MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREM AILAGNQPKENERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKE HLHNVRPDRVLSQVV |
Reference| ID | 1301 |
| Plasmid name | pJM3A |
| GenBank accession number | NZ_CP016737.1 |
| Genome size | 75814 bp |
| Coordinate of oriT [Strand] | 10136..10598 [-] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Lactococcus lactis subsp. cremoris JM3 [1359] |
Reference| [1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072] |