oriTDB

The information of the oriT region

oriTDB accession number	100841
Name	oriT_pJM3A
Sequence Completeness	intact
oriT length	463 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT AGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAA GTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG
IR (inverted repeat)	IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-255] [268-281] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[290-291] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D: 290-291

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGGA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA

121

AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA

181

GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC

241

AACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTAT

301

AGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATACTTATTA

361

CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGAGGCATTTTATCTTTGAGTAA

421

GTTATTGATGGATCAGAAAAATGTATCACAAATTGAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	913
Name	Relaxase_pJM3A
GenBank accession number	WP_011669060
Family	MOB_P
Length	135 aa
UniProt ID	A0A1V0P5P3
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-20, Aligned region: 16..124
Note	putative relaxase
Protein sequence [Download]	MTVIYMPRDTSGVIHSQGQLSRALNYIVNPEKTKGGELVSGQNINVPSNAYNEMLLTREM AILAGNQPKENERFGFHFVQSFSPEDNLTPEQVHEIGLKTMKAYLGESAEFVIATHTDKE HLHNVRPDRVLSQVV

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1301
Plasmid name	pJM3A
GenBank accession number	NZ_CP016737.1
Genome size	75814 bp
Coordinate of oriT [Strand]	10136..10598 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. cremoris JM3 [1359]

Reference

[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]