oriTDB
The information of the oriT region
oriTDB accession number 100839
Name oriT_pUC11D  insolico
Sequence Completeness intact
oriT length 463 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTGCCCCCTTTTCAAGC CACATTGTAATACAAGAACGAAGTGTTTTGTATTACAATGTGATAGCTTGCAGTATTTAT GGATTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG GCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [138-147] [152-161] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [207-219] [223-235] nt  (AAAAGGGGAAAGT..ACTGCCCCCTTTT)
IR3: [241-255] [268-282] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [290-291] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:138-147;R:152-161;L:207-219;R:223-235;L:241-255;R:268-282;D:290-291
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 911
Name Relaxase_pUC11D insolico
GenBank accession number WP_081200409
Family MOBP
Length 410 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.20E-74, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGNKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDISVGSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE
RDTQQRESEIVSLEQAIEPRKSEALKRESTIDRFIDTLKQLAGRVPALTQRVTRKLNQTK
EKILDDFERRFSKDMKNYEKEQQKNLEKQTNRAVQSEKKPTKNHNRGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1299
Plasmid name pUC11D
GenBank accession number NZ_CP016723.1
Genome size 15393 bp
Coordinate of oriT  [Strand] 11764..12226 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis UC11 [1360]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU