oriTDB

The information of the oriT region

oriTDB accession number	100838
Name	oriT_pUC11C
Sequence Completeness	intact
oriT length	462 nt
oriT sequence	AGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAA AAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAA AAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAG CAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCC ACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATG GTTTTATATTTGCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTAC AAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGG CTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [137-146] [151-160] nt (CCCATTTTAT..ATAAAATGGG) IR2: [206-218] [222-234] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [240-254] [267-281] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[289-290] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:137-146;R:151-160;L:206-218;R:222-234;L:240-254;R:267-281;D:289-290

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAA

61

AAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAA

121

AAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAG

181

CAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCC

241

ACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATG

301

GTTTTATATTTGCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTAC

361

AAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGG

421

CTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	910
Name	Relaxase_pUC11C
GenBank accession number	WP_082225095
Family	MOB_P
Length	410 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.90E-75, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDLSVSSYETFKERLSDNGVILSE RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE RDTQQRESEIVNLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1298
Plasmid name	pUC11C
GenBank accession number	NZ_CP016722.1
Genome size	19351 bp
Coordinate of oriT [Strand]	15730..16191 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis UC11 [1360]

Reference

[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]