oriTDB
The information of the oriT region
oriTDB accession number 100838
Name oriT_pUC11C  insolico
Sequence Completeness intact
oriT length 462 nt
oriT sequence AGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAAAA AAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGACAA AAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAAAG CAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGCC ACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATTTATG GTTTTATATTTGCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATTAC AAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAGG CTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [137-146] [151-160] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [206-218] [222-234] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [240-254] [267-281] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [289-290] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:137-146;R:151-160;L:206-218;R:222-234;L:240-254;R:267-281;D:289-290
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 910
Name Relaxase_pUC11C insolico
GenBank accession number WP_082225095
Family MOBP
Length 410 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.90E-75, Aligned region: 9..280
Note putative relaxase
Protein sequence [Download] MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI
DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY
PNHQSAVYTHLDGKNHVLHNHIIVNKVNLETGKKLREQKGESVQRAREMNDRLASRENWH
ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQDLSVSSYETFKERLSDNGVILSE
RGQTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPAITPLE
RDTQQRESEIVNLEQAIEPRKSEALKRESKINRFIDTIKQFAGRVPELTQRVTRKLKQTK
EKILDDFERRFSKDMKNYEQEQQKSLEKQANRDVQSEKKPTKDHDRGMSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1298
Plasmid name pUC11C
GenBank accession number NZ_CP016722.1
Genome size 19351 bp
Coordinate of oriT  [Strand] 15730..16191 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis UC11 [1360]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU