oriTDB
The information of the oriT region
oriTDB accession number 100837
Name oriT_pUC063B  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence TTAGTGGATGATCAAACAAAATACGAGAGTTTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGCAGGTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGTAGTAATTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTTAGGA GGCTATTTATGGATCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [139-148] [153-162] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-256] [269-283] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:139-148;R:153-162;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 909
Name PriA_pUC063B insolico
GenBank accession number WP_081196304
Family MOBP
Length 505 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.40E-49, Aligned region: 16..262
Note putative relaxase
Protein sequence [Download] MTVIYMPKQSNGTVHSAKDLKQLIDYVMNSEKTNDFEYVSGQNILDIHSTCDEMLATRTM
ANALKKKPQKNERFGYHFVQSFSPDDHLTPEQVHEIGCKTMKEYLGSSAEFIIATHTDKP
HLHNHIVLNATDPLTLNKFQQNKNDLERLKEISDNISKEYGCKIIVRPEQKLGNSHKNYL
VYLAKNSYRKEIKNKLEFLVNHSHTWEDFKTKARALNLKVDDTKKYTTYLLEGSEQTKKI
RDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWNDKESIQEKGRKKEIEILLEHWQVT
KETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYIDKRSPANHKIM
VGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ
ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQK
SEQRLEAYQKNQSPKHKARDDDFEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1297
Plasmid name pUC063B
GenBank accession number NZ_CP016716.1
Genome size 44205 bp
Coordinate of oriT  [Strand] 26886..27349 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis UC063 [1360]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU