oriTDB

The information of the oriT region

oriTDB accession number	100836
Name	oriT_p275B
Sequence Completeness	intact
oriT length	464 nt
oriT sequence	TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATTTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA GGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [139-148] [153-162] nt (CCCATTTTAT..ATAAAATGGG) IR2: [208-220] [224-236] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-256] [269-283] nt (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site	[291-292] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:139-148;R:153-162;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC

121

AAAAAGAAAGATTTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAA

181

AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAG

241

CCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTA

301

TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT

361

ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA

421

GGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	908
Name	PriA_p275B
GenBank accession number	WP_081144421
Family	MOB_P
Length	505 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 8.40E-51, Aligned region: 16..262
Note	putative relaxase
Protein sequence [Download]	MTVIKMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTM ANALKNKPRKNERFGYHFVQSFSPDDHLTPEQAHEIGLKTMKEYLGNSAEFIIATHTDKP HLHNHIVLNATDPLTLNKFQQSKNDLERLKEISDNISKEYGCKIIDRPEQKLGNSHKNYL VYLAKNSYRKEIKNKLEFLMNHSHTWEDFKEKARALNLKVDDTKKYTTYLLEGSEQTKKI RDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWKDKESIQEKGQEKEIEILLEHWQVT KETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYIDQRSPANHKIM VGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQK SEQRLEAYQKNQSPKHKARDDDFEI

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1296
Plasmid name	p275B
GenBank accession number	NZ_CP016700.1
Genome size	56332 bp
Coordinate of oriT [Strand]	40221..40684 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactococcus lactis subsp. lactis 275 [1360]

Reference

[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]