oriTDB
The information of the oriT region
oriTDB accession number 100836
Name oriT_p275B  insolico
Sequence Completeness intact
oriT length 464 nt
oriT sequence TTAGTGGATGAACAAACAAAATACGAGAGATTTTTTGTTCGTTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTTCTTCTTTTTGTATGAC AAAAAGAAAGATTTTTTGCCCATTTTATTTTTATAAAATGGGTAGGTGGCGTTTGCGTAA AGCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTAAAACTTCCCCCTTTTCAAG CCACATTGTAATACAAGAACGAAGTGATTTGTATTACAATGTGATAGCTTGCAGTATTTA TGGTTTTATATGGTCTATTTTGTTATAATGATTGTAACCGAATAGGGCGCAATGCTTATT ACAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGA GGCTATTTATGGATCAGAAAAATGTATCACAAAATCAAACAAAG
IR (inverted repeat)IR1: [139-148] [153-162] nt  (CCCATTTTAT..ATAAAATGGG)
IR2: [208-220] [224-236] nt  (AAAAGGGGAAAGT..ACTTCCCCCTTTT)
IR3: [242-256] [269-283] nt  (CACATTGTAATACAA..TTGTATTACAATGTG)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		CTTG|CA
Note predicted by the oriTfinder
L:139-148;R:153-162;L:208-220;R:224-236;L:242-256;R:269-283;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 908
Name PriA_p275B insolico
GenBank accession number WP_081144421
Family MOBP
Length 505 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 8.40E-51, Aligned region: 16..262
Note putative relaxase
Protein sequence [Download] MTVIKMPKQSNGTVHSSKDLNQLIDYVMNPEKTNDFEYVSGQNILDIHSTCDEMLATRTM
ANALKNKPRKNERFGYHFVQSFSPDDHLTPEQAHEIGLKTMKEYLGNSAEFIIATHTDKP
HLHNHIVLNATDPLTLNKFQQSKNDLERLKEISDNISKEYGCKIIDRPEQKLGNSHKNYL
VYLAKNSYRKEIKNKLEFLMNHSHTWEDFKEKARALNLKVDDTKKYTTYLLEGSEQTKKI
RDRSLKNDKFLKENLKERIEKNTIGYSVEEVVKLWKDKESIQEKGQEKEIEILLEHWQVT
KETEKDLVVTIDTAFDNEATIKIPARCVDKLENGQYKIFIKKGDRFSYIDQRSPANHKIM
VGATVAKNLQRQSGNIPLYSDNVNIKLKQVFHEFDFLISQGLGFDRSFETIGEELKATYQ
ETQHQLDKLDTKILEYVETTKTLPYEDTSIRDTIKNLTKERDDLRDTLYKVDKNIQYYQK
SEQRLEAYQKNQSPKHKARDDDFEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1296
Plasmid name p275B
GenBank accession number NZ_CP016700.1
Genome size 56332 bp
Coordinate of oriT  [Strand] 40221..40684 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Lactococcus lactis subsp. lactis 275 [1360]
Reference
[1] Kelleher P et al (2017) Comparative and functional genomics of the Lactococcus lactis taxon; insights into evolution and niche adaptation. BMC Genomics. 18(1):267. [PMID:28356072]

Microbial Bioinformatics Group in MML, SJTU