oriTDB

The information of the oriT region

oriTDB accession number	100835
Name	oriT_pNCI002
Sequence Completeness	core
oriT length	34 nt
oriT sequence	GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG
IR (inverted repeat)	[4-18] [22-34] nt (CCCATTTATACCCCT..AGGGGTAAATGGG)
Location of nic site	[2-3]nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:4-18;R:22-34;D:2-3

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGCCCATTTATACCCCTTTCAGGGGTAAATGGG

Reference
N/A

I. Information of Relaxase

ID	907
Name	Nickase_pNCI002
GenBank accession number	WP_042385886
Family	MOB_Q
Length	643 aa
UniProt ID	_
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 2.20E-71, Aligned region: 16..223
Note	putative relaxase
Protein sequence [Download]	MAYFLFRTQIISKSNRSAVACAAYRSGEALYSERDGLTKKYGHRDVPPETYILAPKHAPE WVYNRERLWNEVEKVEKQHNAQLAREIIVALPTQLTNEEQTQLLLDFCKENFADEGMVAD IAIHRDKKHNPHAHIMLTMRPFNEDGTWGKKRKKVNGKSVHLTNWNDKETLLKWRKNFAD RINEKFKEKGIDDRVSHESYEKQGIDKVPQIRLSREAYQYEQRMKREAEKQGKPYEPVTY YGKLNQEIQRINQELKALKQKEKVVSISDYQEEKSLNETIESIRKNASLNDVEKASLLLV AKRAKTYVDFAVARRVYHDLAEGNWKKKIDSQQLKLRALKNVINKAYHVYQQEPKQVIQY GFHPGKFNEQLREKIAQLKEMEKSFENELTKYEAVLKKAELALEVQKRLTEEEFRVLYPH AGADFRIEEKYHAVQYFKDTGGVLPESDIPAYAAKKEQEIHRMPTFAEQTRNMYQSLFVL NRTIQKQSKERMEALKARDFEQAFEASRKIEQCMLRKEKMSKELEENKAVLQAILSEHYG RDVSITNTAALLRLHELVERGESSKDLEADLRRIHVSEQQKLERMPKPETEAQIMEYQYR QHVADGLLEAIEEAQRANDRKKYEKDPTKKRQRRRYRGQEFER

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1295
Plasmid name	pNCI002
GenBank accession number	NZ_CP016624.1
Genome size	47893 bp
Coordinate of oriT [Strand]	12520..12553 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Parageobacillus thermoglucosidans NCIMB 11955 [1426]

Reference
N/A