oriTDB

The information of the oriT region

oriTDB accession number	100811
Name	oriT_pSLy5
Sequence Completeness	intact
oriT length	174 nt
oriT sequence	GGTGTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAT TATGTAGGCACGGGTGCGGGAATCAGTGAAGTGCTTCATGTGGCAGGAGGAAAATGCAGC AGCAGAGCGTAAGCGGAATATATGAGACAGGAGATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTGTCGGGGC..GCCCTGAACC)
Location of nic site	[40-41] nt
Conserved sequence flanking the nic site	CTAG\|CGGA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:40-41

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTGTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAT

61

TATGTAGGCACGGGTGCGGGAATCAGTGAAGTGCTTCATGTGGCAGGAGGAAAATGCAGC

121

AGCAGAGCGTAAGCGGAATATATGAGACAGGAGATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	883
Name	Nuclease_pSLy5
GenBank accession number	WP_000635804
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1271
Plasmid name	pSLy5
GenBank accession number	NZ_CP015917.1
Incompatibility group	ColRNAI
Genome size	8972 bp
Coordinate of oriT [Strand]	8024..8197 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 210205630 [562]

Reference
N/A