oriTDB

The information of the oriT region

oriTDB accession number	100806
Name	oriT_pShin-10
Sequence Completeness	core
oriT length	40 nt
oriT sequence	TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGC
IR (inverted repeat)	[16-24] [29-37] nt (ATACGTCGC..GCGACGCCT)
Location of nic site	[8-9] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:16-24;R:29-37;D:8-9

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCCAAGGGCGCAATTATACGTCGCTGACGCGACGCCTTGC

Reference
N/A

I. Information of Relaxase

ID	878
Name	TraA_pShin-10
GenBank accession number	WP_064334768
Family	MOB_Q
Length	1193 aa
UniProt ID	A0A1A9GEW8
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 3.10E-73, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 5.90E-57, Aligned region: 381..570 AAA_19 [PF13245.5], Evalue: 2.30E-10, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 6.70E-05, Aligned region: 666..737
Note	putative relaxase
Protein sequence [Download]	MAVPHFSVSVVARGSGRSAVLSAAYRHCVKMEFEREARTIDYTRKQGLLHEEFVIPEDAP DWLRSMIADRSVAAASEAFWNAVEGFEKRSDAQLAKDVTMALPIELSADQNIALVREFVA SHITAKGMVADWVYHDAPGNPHVHLMTTLRPLTDDGFGAKKVAVLGADGKSLRNDSGKIV YELWAGGVGDFNAFRDGWFVCQNRHLALAGLDIRVDGRSYEKQGIALEPTIHLGVGANAI KQKSAEASEPITLERIELQEERRAENARRIEDRPAVVIDMIAREKSVFDERDVAKILHRY VDDAGLFQSLMARVLQSPDVVRLEREHVDFASGARVPAKLTTRDLIRTEAEMASRAVFLA GRSSHRVENAILQETFARYSRLSKEQKTAIAHVAGSHRIAAVVGRAGAGKTTMMKAAREA WEAAGYRVVGGALAGKAAEGLETEAGIVSRTLSSWELRWNDGRSQLDDKTVFVLDEAGMV SSRQLALFVEAVTKAGAKLVLVGDPDQLQPIEAGAAFRAIADRIGYAELETIYRQREQWM RDASLDLARGNVAAALSAYDAADLVQTGWTRQDAIEGLIDNWNYDFDPTKTTLILAHRRR DVRELNELARAKLVERGIVGEGSVFRTEDGARRFAAGDQIVFLKNEGSLGVKNGMLAKVV EAAPGRIVAEIGEGDSRRQVTVEQRFYSNVDHGYATTIHKSQGATVDRVKVLASNTLDRH LTYVAMTRHREAAELYVGMEEFTNRRGGVLVDHGEAPYEHKPGNRDSYFVTLTYGDGQER TIWGVDLARAIDEADAHVGDRIGLRHEGSESVRLPDGTMAKRNTWKIVPVKELAMERLKE RLSRDASKETTLDYEGASSYRAALRFAEARGMHLMNVARTISRDRLAWTVRQKQKLVDLA ARLKAVGAALGLARGSDKQSVPTIVKEARPMVAGITTFAKSLEQSVEDKLSNDPALKQQW KDVSTRFQMVYAQPEAAFAAINVDAMIKDQARATATIAKISAEPETFGTLKGKTGMFASR ADKQERDTALVNAPALARNLERYLRGRAEAERKHETEERAARIKVSVDIPALSPNAKQTL ERIRDAIDRNDLPAGLEYALADKMVKAELEGFAKAVTERFGERTFLGVSAKEAGGEAFKT VTRGMTDPQKAEVQTAWNSMRAVQQLAAHERVVVGIKQAEALRQTQAKGLSLK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1266
Plasmid name	pShin-10
GenBank accession number	NZ_CP015746.1
Genome size	112708 bp
Coordinate of oriT [Strand]	24514..24553 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Shinella sp. HZN7 [879274]

Reference

[1] Qiu J et al (2016) The Complete Genome Sequence of the Nicotine-Degrading Bacterium Shinella sp HZN7. Front Microbiol. 1.227777778. [PMID:27625640]