oriTDB

The information of the oriT region

oriTDB accession number	100805
Name	oriT_pShin-05
Sequence Completeness	core
oriT length	31 nt
oriT sequence	AGGGCGCAATTATACGTCGCTGATGCGACGC
IR (inverted repeat)	[15-20] [25-30] nt (CGTCGC..GCGACG)
Location of nic site	[4-5] nt
Conserved sequence flanking the nic site	AGGG\|CGCA
Note	predicted by the oriTfinder
	L:15-20;R:25-30;D:4-5

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGGGCGCAATTATACGTCGCTGATGCGACGC

Reference
N/A

I. Information of Relaxase

ID	877
Name	TraA_pShin-05
GenBank accession number	WP_064334296
Family	MOB_Q
Length	1102 aa
UniProt ID	A0A1A9GDD3
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 1.80E-73, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 8.50E-57, Aligned region: 381..569 AAA_19 [PF13245.5], Evalue: 1.60E-10, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 3.80E-05, Aligned region: 656..737
Note	putative relaxase
Protein sequence [Download]	MAVPHFSVSIVARGSGRSAVLSAAYRHCAKMDYEREARTIDYTRKQDLLHEEFVIPVDAP EWLQSMIADRSVSGASEAFWNKVEKFEKRSDAQLAKDVTIALPIELSADKNIELVRDFVA RHITAEGMVADWVFHDAPGNPHIHLMTTLRPLTEDGFGSKKVAVVGPDGKALRNDSGKIV YELWAGGLDDFNAFRDGWFACQNRHLALACLDIRIDGRSFEKQGIELTPTIHLGVGTKAI ERKAEGAEEKAALERLELQEERRAENARRIQRDPEIVLDLIMREKSVFDERDVAKILHRN IDDAGLFQNLMARILQSPDTLRLDCERLDFVTGMRAPAKYTTRELIRLEAEMANRAIWLS QRSSYGVRKTVLEATFARHERLSDEQRAAIEHVASGERIAAVIGLAGAGKTTMMKAAREA WEAAGYRVVGGALAGKAAEGLEKEAGIVSRTLSSWELRWNQGRGKLNDKTVFVLDEAGMV SSRQMALFVETVTRAGAKLVLVGDPEQLQPIEAGAAFRAIADRIGYVELETIYRQREQWM RDASLDLARGNVGKAVSAYQSNGKMIGSELKADAVTNLIADWNRDYDPAKSTLILAHLRR DVRMLNELARKKLIERGIIEHGSPFKTADGVRSFAAGDQIVFLKNEGSIGVKNGMLGKVV EAASGRIVAVIGEGEHLRQVSVEQRFYTNVDHGYATTVHKSQGATVDRVKVLASVSLDRH LTYVAMTRHREDLGVYYGSRSFAKAGGLVEILSRRNSKETTLDYAGGSIYGQALRFAEAR GMHVVNVARTVARDRLEWTARQKQKLVDLAGRLAVLGARLGFATSAASTIQTSAKEANPM VAGITTFPKSIDQAVEDKLASDPALKKQWEEVSMRFHHVYARPEAAFKAVDVAAMLRDQA AAAKTIARIASEPETFGALKGKTGVFASRADKSERDRALKNVTPLADSISDYLRQRGDAE RRNHAEELAVRRQVALEIPALSSNAKSVLERVRDSIDRNDLPSGLEYALADKMVKAELEG FAKAVSERFGERTFLPLAAKDANGEAFQRVTSGMNAAQKSEVQQGWTTMRTVQQLSAHER SVTALKQTEALRQTRSQGLTLK

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1265
Plasmid name	pShin-05
GenBank accession number	NZ_CP015741.1
Genome size	155026 bp
Coordinate of oriT [Strand]	90914..90944 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Shinella sp. HZN7 [879274]

Reference

[1] Qiu J et al (2016) The Complete Genome Sequence of the Nicotine-Degrading Bacterium Shinella sp HZN7. Front Microbiol. 1.227777778. [PMID:27625640]