oriTDB
The information of the oriT region
oriTDB accession number 100804
Name oriT_pSKGH01-5  insolico
Sequence Completeness intact
oriT length 475 nt
oriT sequence ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACGAATCT AGCATATATGCGGCTTAATTACACATGTGTAATACGTTGTGTATTACACATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTG CAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAAC GGACGGGAATTAGACAAAAATAAGACACGAGGAGTAAGTTATTGAGACAAGAAAAGGACA CAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA
IR (inverted repeat)IR1: [145-155] [161-171] nt  (ATGTGTAATAC..GTATTACACAT)
IR2: [208-218] [223-233] nt  (GCATTTACGAT..ATCGTAATTGC)
IR3: [335-344] [347-356] nt  (CCTTTCATGC..GCATGAAAGG)
Location of nic site [242-243] nt
Conserved sequence flanking the
  nic site 		GGTGT|ATAGC
Note predicted by the oriTfinder
L:145-155;R:161-171;L:208-218;R:223-233;L:335-344;R:347-356;D:242-243
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 876
Name Relaxase_pSKGH01-5 insolico
GenBank accession number WP_000932317
Family MOBF
Length 969 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 5.70E-80, Aligned region: 12..288
AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659
Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..654
Viral_helicase1 [PF01443.17], Evalue: 2.30E-06, Aligned region: 780..897
UvrD_C_2 [PF13538.5], Evalue: 2.10E-09, Aligned region: 849..896
Note putative relaxase
Protein sequence [Download] MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD
GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEE
AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL
TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE
AELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA
ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD
DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ
LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG
LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK
AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH
DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQAELGLKGQGINYPLLNRLDT
TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR
FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE
KKLDKAASREGFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKN
NDERAFTSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1264
Plasmid name pSKGH01-5
GenBank accession number NZ_CP015505.1
Genome size 34611 bp
Coordinate of oriT  [Strand] 12479..12953 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae SKGH01 [573]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU