oriTDB

The information of the oriT region

oriTDB accession number	100804
Name	oriT_pSKGH01-5
Sequence Completeness	intact
oriT length	475 nt
oriT sequence	ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACGAATCT AGCATATATGCGGCTTAATTACACATGTGTAATACGTTGTGTATTACACATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTG CAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAAC GGACGGGAATTAGACAAAAATAAGACACGAGGAGTAAGTTATTGAGACAAGAAAAGGACA CAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA
IR (inverted repeat)	IR1: [145-155] [161-171] nt (ATGTGTAATAC..GTATTACACAT) IR2: [208-218] [223-233] nt (GCATTTACGAT..ATCGTAATTGC) IR3: [335-344] [347-356] nt (CCTTTCATGC..GCATGAAAGG)
Location of nic site	[242-243] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	predicted by the oriTfinder
	L:145-155;R:161-171;L:208-218;R:223-233;L:335-344;R:347-356;D:242-243

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCGCTTCGGTCACTC

61

TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACCGATGTAATACATACGAATCT

121

AGCATATATGCGGCTTAATTACACATGTGTAATACGTTGTGTATTACACATTAAAACACA

181

AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAAATCGTAATTGCGTATGGT

241

GTATAGCCGTTAAGGGATACCATACCACGCCTTTTTTAAGGGAGAAACCGGTGTTACGTG

301

CAAGTGAATCGCTCAAAAAGCGTTCACATTCACACCTTTCATGCTTGCATGAAAGGAAAC

361

GGACGGGAATTAGACAAAAATAAGACACGAGGAGTAAGTTATTGAGACAAGAAAAGGACA

421

CAAATAAGACATTTTTTAGAAAAAAACATTGACTTGAGACTAGAAATGGACAATA

Reference
N/A

I. Information of Relaxase

ID	876
Name	Relaxase_pSKGH01-5
GenBank accession number	WP_000932317
Family	MOB_F
Length	969 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 5.70E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659 Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..654 Viral_helicase1 [PF01443.17], Evalue: 2.30E-06, Aligned region: 780..897 UvrD_C_2 [PF13538.5], Evalue: 2.10E-09, Aligned region: 849..896
Note	putative relaxase
Protein sequence [Download]	MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVTLQALVNGDKRIIEAHDRAVTKAIEE AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE AELAKNGLSRETASHEQKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRNKDEARKLVD KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINAYIQAELGLKGQGINYPLLNRLDT TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKGVQSGEVLLENEKGRLVKID ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE KKLDKAASREGFKTAALELEQLKRYAKEQQHDAREKGRDKTEPTQEKDNAKRKGKGHDKN NDERAFTSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1264
Plasmid name	pSKGH01-5
GenBank accession number	NZ_CP015505.1
Genome size	34611 bp
Coordinate of oriT [Strand]	12479..12953 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae SKGH01 [573]

Reference
N/A