oriTDB
The information of the oriT region
oriTDB accession number 100800
Name oriT_p2011C-3911-1  insolico
Sequence Completeness intact
oriT length 279 nt
oriT sequence CGCTAGCAGCGCCCCTGGCGGTATCCTATAAAAAAATACACCGCGCCGCTAGCAGCACCC CTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCG GCGCGTTGCTGTAGCGGCGTTAACAACGCCGCTTTAAATAAATCAGATTTAAACAATATA AATCCACAAATACAACTCAATGATATTAAACGCAAATCAGCAAAAACTTGTTTTTGCGTG GGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
IR (inverted repeat)[220-227] [230-237] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [246-247] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:220-227;R:230-237;D:246-247
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 872
Name TraI_p2011C-3911-1 insolico
GenBank accession number WP_032326999
Family MOBF
Length 1756 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 4.80E-75, Aligned region: 10..284
TraI [PF07057.10], Evalue: 1.30E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAADQRAE
TRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPEAGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVIMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVADSPFTALKLENGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGHDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGEASLESAISHQKSALHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTG
FTELGGEINAQIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK
QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGTIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQME
RDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1260
Plasmid name p2011C-3911-1
GenBank accession number NZ_CP015239.1
Incompatibility group IncFIB(AP001918)
Genome size 125977 bp
Coordinate of oriT  [Strand] 92474..92752 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli 2011C-3911 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU