oriTDB

The information of the oriT region

oriTDB accession number	100800
Name	oriT_p2011C-3911-1
Sequence Completeness	intact
oriT length	279 nt
oriT sequence	CGCTAGCAGCGCCCCTGGCGGTATCCTATAAAAAAATACACCGCGCCGCTAGCAGCACCC CTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCG GCGCGTTGCTGTAGCGGCGTTAACAACGCCGCTTTAAATAAATCAGATTTAAACAATATA AATCCACAAATACAACTCAATGATATTAAACGCAAATCAGCAAAAACTTGTTTTTGCGTG GGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT
IR (inverted repeat)	[220-227] [230-237] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[246-247] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:220-227;R:230-237;D:246-247

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGCTAGCAGCGCCCCTGGCGGTATCCTATAAAAAAATACACCGCGCCGCTAGCAGCACCC

61

CTAATATAAAATAATGTTTTTTATAAAAATAGTCAGTACCACCCCTACAAAGCGGTGTCG

121

GCGCGTTGCTGTAGCGGCGTTAACAACGCCGCTTTAAATAAATCAGATTTAAACAATATA

181

AATCCACAAATACAACTCAATGATATTAAACGCAAATCAGCAAAAACTTGTTTTTGCGTG

241

GGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTT

Reference
N/A

I. Information of Relaxase

ID	872
Name	TraI_p2011C-3911-1
GenBank accession number	WP_032326999
Family	MOB_F
Length	1756 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 4.80E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 1.30E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAADQRAE TRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPEAGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVIMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVADSPFTALKLENGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGHDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGEASLESAISHQKSALHTPAQQAIHLALPVVESKNLAFSMVDLLTEAKSFAAEGTG FTELGGEINAQIKRGDLLHVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGTIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1260
Plasmid name	p2011C-3911-1
GenBank accession number	NZ_CP015239.1
Incompatibility group	IncFIB(AP001918)
Genome size	125977 bp
Coordinate of oriT [Strand]	92474..92752 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli 2011C-3911 [562]

Reference
N/A