oriTDB
The information of the oriT region
oriTDB accession number 100778
Name oriT_pUSA06-1-SUR11  insolico
Sequence Completeness core
oriT length 53 nt
oriT sequence CCAGCAAGCCGGTATATTCAGAACGAAGTGGCTAGAATATACGACGCTTGCCA
IR (inverted repeat)[12-19] [35-42] nt  (GTATATTC..GAATATAC)
Location of nic site [50-51] nt
Conserved sequence flanking the
  nic site 		CGCTTG|CCA
Note predicted by the oriTfinder
L:12-19;R:35-42;D:50-51
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 850
Name Rlx_pUSA06-1-SUR11 insolico
GenBank accession number WP_000246202
Family MOBP
Length 320 aa
UniProt ID A0A0U2YPT6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 5.80E-79, Aligned region: 8..238
Note putative relaxase
Protein sequence [Download] MATTKLGNTKSASRAINYAEERAEEKSGLNCDVDYAKSYFKQTRALYGKENGVQAHTVIQ
SFKPGEVTAKECNEIGLELAKKIAPDYQVAVYTHTDKDHYHNHIIINSVNLETGNKYQSN
KEQRDFIKKANDQLCEERGLSVPEKSSEIRYTLAEQNMIDKDKRSWKNDIRMAVEETKDN
AVDFEEFNTLLKEKGVEITRVTKNNVTYRHIEEDKKVRGNKLGDSYDKGVIENGFAIEKF
RREREEEREYDEYADTFEVDWDAVAENSEDLRKRRIARTEETKQASNKIYIRDERTTGLE
RKGIAGNQVEFEKDDGGLSR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1238
Plasmid name pUSA06-1-SUR11
GenBank accession number NZ_CP014406.1
Genome size 4397 bp
Coordinate of oriT  [Strand] 4022..4074 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Staphylococcus aureus USA300-SUR11 [1280]
Reference
[1] Sabat AJ et al (2017) Complete-genome sequencing elucidates outbreak dynamics of CA-MRSA USA300 (ST8-spa t008) in an academic hospital of Paramaribo, Republic of Suriname. Sci Rep. 7:41050. [PMID:28106148]

Microbial Bioinformatics Group in MML, SJTU