oriTDB
The information of the oriT region
oriTDB accession number 100772
Name oriT_pJJ1887-4  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site
GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 844
Name TraI_pJJ1887-4 insolico
GenBank accession number WP_074159540
Family Other
Length 1370 aa
UniProt ID _
PDB ID _
Pfam TraI [PF07057.10], Evalue: 1.30E-56, Aligned region: 1048..1170
AAA_30 [PF13604.5], Evalue: 3.60E-34, Aligned region: 582..773
AAA_19 [PF13245.5], Evalue: 8.00E-09, Aligned region: 587..722
Note putative relaxase
Protein sequence [Download] MRQKAASSLTLQQCQQNRVTVHPEKSVPRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQR
ERVAELVMMAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLLEGMAFTPGSTVI
VDQGEKLSLKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQR
PATIISEPDRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGH
PEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDA
QGETQVVRISSLDSSWSLFRPEKMPVADGERLRVTGKIPGLRVSGGDRLQVASVSEDAMT
VVVPGRAEPASLPVSDSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLA
RSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQIKARAGETLLETAISLQKAGLHTPAQQ
AIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGY
GTGLLVSRASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILETSDR
FTVVQGYAGVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASF
LHDTQLQQRSGETPDFSNTLFLLDESSMVGNTEMARAYALIAAGGGRAVASGDTDQLQAI
APGQPFRLQQTRSAADVVIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRL
EGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEAFPDIPMTLYEAIVRDYTGRTPEAREQ
TLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDA
LALVDNVYHRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMR
FTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQ
GASETFAIALEGTEGNRKLMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTA
HDVLEPKPDREVMNAQRLFSTARELRDVAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPY
VALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGEGRVKGSGDAQFVALQGSRNGESLLAD
NMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAE
VLAQRQAEEAIRRETERRADEIVRKMAENKPDLPDGKTELAVRDIAGQERDRSAISERET
ALPESVLRESQREREAVREVARENLLQERLQQMERDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1232
Plasmid name pJJ1887-4
GenBank accession number NZ_CP014321.1
Incompatibility group IncFIA
Genome size 107507 bp
Coordinate of oriT  [Strand] 28313..28762 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli JJ1887 [1355101]
Reference
[1] Johnson TJ et al (2016) Complete Genome Sequence of a CTX-M-15-Producing Escherichia coli Strain from the H30Rx Subclone of Sequence Type 131 from a Patient with Recurrent Urinary Tract Infections, Closely Related to a Lethal Urosepsis Isolate from the Patient's Sister. Genome Announc. 4(3). [PMID:27174264]