oriTDB
The information of the oriT region
oriTDB accession number 100769
Name oriT_pKpN06-COL  insolico
Sequence Completeness intact
oriT length 193 nt
oriT sequence GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCG GAGTGTATACTGGCTAAACATGTTATGACGGAGTGGTAATTTATGAAAGTGCTTCATGTG ACAGGGGAAAAATGCGGCTGCCGGGCGTAAGTGCAAAATGCAGGTGGTGATGAAATGACG CCTCCTCGCTCAC
IR (inverted repeat)[22-29] [33-40] nt  (TTCGGGGC..GCCCTGAA)
Location of nic site [73-74] nt
Conserved sequence flanking the
  nic site 		CTGG|CTAA
Note predicted by the oriTfinder
L:22-29;R:33-40;D:73-74
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 841
Name Putative relaxase of pKpN06-COL insolico
GenBank accession number WP_045265544
Family MOBP
Length 497 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 2.80E-17, Aligned region: 42..273
Note putative relaxase
Protein sequence [Download] MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSF
AEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRL
QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLA
LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL
RAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERDA
AHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHAVREEQRRTALD
LRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQ
KALELERSQWQHHGPSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1229
Plasmid name pKpN06-COL
GenBank accession number NZ_CP014305.2
Genome size 3223 bp
Coordinate of oriT  [Strand] 2485..2677 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Klebsiella pneumoniae KpN06 [573]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU