oriTDB
The information of the oriT region
oriTDB accession number 100760
Name oriT_puk_P46212  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site
GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 832
Name Relaxase_puk_P46212 insolico
GenBank accession number WP_000130000
Family MOBC
Length 101 aa
UniProt ID _
PDB ID _
Pfam PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85
HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94
Note putative relaxase
Protein sequence [Download] MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS
RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1220
Plasmid name puk_P46212
GenBank accession number NZ_CP013657.1
Incompatibility group IncFIA
Genome size 143748 bp
Coordinate of oriT  [Strand] 73215..73664 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli uk_P46212 [562]
Reference
[1] Stoesser N et al (2016) Evolutionary History of the Global Emergence of the Escherichia coli Epidemic Clone ST131. MBio. 7(2):e02162. [PMID:27006459]