oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100739
Name	oriT_pSF-468-3
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	811
Name	Nuclease_pSF-468-3
GenBank accession number	WP_000635804
Family	MOB_P
Length	517 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.50E-20, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLQGNPEEVRELIDATPFAKKYTSGVLSF AEKELPPGGREKVMASFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELQTGKRL QPYYDRADRPRIDAWQTLVNHHYGLHDPNAPENRRTLTLPDNLPETKQALAEGVTRGIDA LYHAGEIKGRQDVIQALTEAGLEVVRVTRSSISIADPNGGKNIRLKGAFYEQSFADGRGV REKAERESRIYRENAEQRVQEARRICKRGCDIKRDENQRRYSPVHSLDRGIAGKTPGRGE RGDDAAQEGRVKAGREYGHDVTGDSLSPVYREWRDALVSWREDTGEPGRNQEAGRDIAET EREDMGRGVCAGREQEIPCPSVREISGGDSLSGERVGTSEGVTQSDRAGNTFAERLRAAA TGLYAAAERMGERLRGIAEDVFAYATGQRDAERAGHAVESAGAALERADRTLEPVIQREL EIREERLIQEREHVLSLERERQPEIQERTLDGPSLGW

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1199
Plasmid name	pSF-468-3
GenBank accession number	NZ_CP012628.1
Incompatibility group	ColRNAI
Genome size	6647 bp
Coordinate of oriT [Strand]	1179..1261 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli SF-468 [562]

Reference

[1] Stephens CM et al (2015) Complete Genome Sequences of Four Escherichia coli ST95 Isolates from Bloodstream Infections. Genome Announc. 3(6). [PMID:26543109]