oriTDB

The information of the oriT region

oriTDB accession number	100738
Name	oriT_pSF-468-2
Sequence Completeness	intact
oriT length	103 nt
oriT sequence	TCAGCTCCTTACTGGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCG CGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTCAGG
IR (inverted repeat)	IR1: [19-26] [30-37] nt (GTCGGGGC..GCCCTGAC) IR2: [44-58] [67-81] nt (GCAATTGTATAATCG..CGGTTATACAATTGC)
Location of nic site	[89-90] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:19-26;R:30-37;L:44-58;R:67-81;D:89-90

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TCAGCTCCTTACTGGGGTGTCGGGGCGAAGCCCTGACCAGGAGGCAATTGTATAATCGCG

61

CGTGCGCGGTTATACAATTGCACATCCTGTCCCGTTTTTCAGG

Reference
N/A

I. Information of Relaxase

ID	810
Name	Putative relaxase of pSF-468-2
GenBank accession number	WP_021520371
Family	MOB_P
Length	899 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.20E-64, Aligned region: 47..315
Note	putative relaxase
Protein sequence [Download]	MNAVIPKKRRDGKSSFEDLVSYVSVRDDMTDEELDLSSSSQAEQPHRSRFSRLVDYATRL RNESFVALVDVMKDGCEWVNFYGVTCFHNCTSLETAAADMEYIARQAHYAKDDTDPVFHY ILSWQSHESPRPEQIYDSVRHTLKSLGLADHQYVSAVHTDTDNLHVHVAVNRVHPETGYL NRLSWSQEKLSRACRELELKHGFAPDNGCWVHAPGNRIVRKTAVERDRQNAWTRGKKQTF REYVAQTAVAGLRSEPVNDWLSLHRRLAEDGLYLSQMDGKFLVMDGWDRNREGVQLDSFG PSWCAEKLMKKMGDYTPVPKDIFSQVEAPGRYNPDFIAADVRPEKIAETESLQQYACRHL GERLPEMAREGRLENCQAIHRTLAEAGLWMRVQHGHLVICDGYDHNQTPVRADSVWSLLT LDNVNQLDGGWQPVPTDIFLQVTPTERFRGRRMESCPATDKEWHRMRTGTGPQGAIKREL FSDKESLWGYSISHCSPQIEEMITQGEFTWQRCHELFAQQGLMLQKQHHGLVVVDAFNHE QTPVKASSIHPDLTLGRAEPQAGPFVSAPADLFDRVQPESRYNPELAVSDRYGVSSKRDP MLRRQRREARAEARADLRARYLAWREQWRKPDLRYGERCREIHQACRLRKSHIRAQYDDP ALRKLHYHIAEVQRMQALIRLKEDIRNERQKLIADGKWYPPSYRQWVEIQAAQGDRAAVS QLRGWDYRDRRKDKSRTTTTDRCVVLCEPGGTPVYGNTGDLEARLQKNGSVRFRDRRTGE FVCTDYGDRVVFRNHHDRNALADKLDLIAPVLFGRDPRMGFEPEGNDKQFNQVFAEMVAW HNVTGRTGHEDYRITRPDVDHHREGSERYYRDYIAANSNDDASLPPPEQDKRWEPPSPG

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1198
Plasmid name	pSF-468-2
GenBank accession number	NZ_CP012627.1
Incompatibility group	IncI1
Genome size	92766 bp
Coordinate of oriT [Strand]	66217..66319 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli SF-468 [562]

Reference

[1] Stephens CM et al (2015) Complete Genome Sequences of Four Escherichia coli ST95 Isolates from Bloodstream Infections. Genome Announc. 3(6). [PMID:26543109]