oriTDB
The information of the oriT region
oriTDB accession number 100710
Name oriT_pC227-11-1  insolico
Sequence Completeness intact
oriT length 450 nt
oriT sequence ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAATGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGCGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG CAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCAATCT
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACCAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 782
Name TraI_pC227-11-1 insolico
GenBank accession number WP_001390493
Family Other
Length 1231 aa
UniProt ID _
PDB ID _
Pfam TraI [PF07057.10], Evalue: 1.70E-55, Aligned region: 980..1102
AAA_30 [PF13604.5], Evalue: 4.00E-34, Aligned region: 514..705
AAA_19 [PF13245.5], Evalue: 8.80E-09, Aligned region: 519..654
Note putative relaxase
Protein sequence [Download] MAREQGREVQIIAADRRSQMNLKQDERLSGELITGRRQLQDGMTFTPGSTVIVDQGEKLS
LKETLTLLDGAARHNVQVLITDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEP
DRNVRYARLAGDFAASVKAGEESVAQVSGVREQAILTQAIRSELKTQGVLGHPEVTMTAL
SPVWLDSRSRYLRDMYRPGMVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVR
ISSLDSSWSLFRPEKMPVADGERLRVTGKTPGLRVSGGDRLQVASVSEDAMTVVVPGRAE
PATLPVADSPFTALKLENGWVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRL
YSSLDETRTAEKLARHPSFTVVSEQIKARAGETSLETAISLQKAGLHTPAQQAIHLALPV
LESKNLAFSMVDLLTEAKSFAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSR
ASYEAEKSILRHILEGKEAVTPLMERVPGELMETLTSGQRAATRMILEASDRFTVVQGYA
GVGKTTQFRAVMSAVNMLPASERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQ
RSGETPDFSNTLFLLDESSMVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRL
QQTRSAADVAIMKEIVRQTPELREAVYSLINRDVERALSGLESVKPSQVPRLEGAWAPEH
SVTEFSHSQEAKLAEAQQKAMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLN
EDRRVLNSMIHDAREKAGELGKEQVMVPVLNTANIRDGELRRLSTWEKNPDALALVDSVY
HRIAGISKDDGLITLQDAEGNTRLISPREAVAEGVTLYTPDKIRVGTGDRMRFTKSDRER
GYVANSVWTVTAVSGDSVTLSDGQQTRVIRPGQERAEQHIDLAYAITAHGAQGASETFAI
ALEGTEGNRKQMAGFESAYVALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKP
DREVMNAQRLFSTARELRDVAAGRAVLRQAGLSGGDSPARFIAPGRKYPQPYVALPAFDR
NGRSAGIWLNPLTTDDGNGLRGFSGEGRVKGSEDAQFVALQGSRNGESLLADNMQDGVRI
ARDNPDSGVVVRIAGEGRPWNPGAITGGRVWGDIPDSSVQPGAGNGEPVTAEVLAQRQAE
EAIRRETERRADEIVRKMAENKPDLPDGKTE
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1170
Plasmid name pC227-11-1
GenBank accession number NZ_CP011332.1
Incompatibility group IncFIB(AP001918)
Genome size 75079 bp
Coordinate of oriT  [Strand] 17000..17449 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O104:H4 str. C227-11 [1048254]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU