oriTDB

The information of the oriT region

oriTDB accession number	100706
Name	oriT_pVR50C
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ATCATTTGGCATCAGTGAGGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCATGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCATGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

ATCATTTGGCATCAGTGAGGATT

Reference
N/A

I. Information of Relaxase

ID	778
Name	Putative relaxase of pVR50C
GenBank accession number	WP_046072957
Family	MOB_P
Length	532 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.40E-18, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGARVLRGAPEEVRELIDATPFAKKYTSGVLSF AEQTLPPGERERVMESFERVLMPGLEKNQYSILWVEHQDKGRLELNFVIPNMELASGKRL QPYYDRADRPRINAWQTLVNHHYGLHDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYHAGALKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGDRL REETERACRLYREHAEQRVQEARSVCRQGTELKRAENQRRYQRTHSSGERTTGDAEQCRE RAVQRHRERTGPAAGRAALEAGQRADTVPVADGALRHGRDFSGVMGADSVAGKPDCGEHP GYQGPGRDAGETPGKNMGCDVSRGQEREISGVAVRGEGGKQLDGGEEERSKTGQGVSEYD RAGNALTERLRAIAAGLYAAAERLGERLHGFAEYVRADTTGERATEGTVRRLESAGAEIS RAGGPLESVIYHEQQIRDEQERQARLERERARSWHEEQEREARVWRGPSIGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1166
Plasmid name	pVR50C
GenBank accession number	NZ_CP011137.1
Incompatibility group	ColRNAI
Genome size	6989 bp
Coordinate of oriT [Strand]	4119..4201 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli VR50 [941323]

Reference

[1] Beatson SA et al (2015) Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition. Infect Immun. 83(5):1749-64. [PMID:25667270]