oriTDB
The information of the oriT region
oriTDB accession number 100703
Name oriT_pCI5  insolico
Sequence Completeness intact
oriT length 542 nt
oriT sequence TTACTCTGGCCACAAGATAAAACCTTTCATCATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAATAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGTGCCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGGGTCGCTATAGGGTCGTAACAGGATCGCTAACGACTC TTTATTTAATAATTCAGAATTATTAAATATAAATAGCATTTGTTAATTGCATGATTTAAA ACACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCC AGGCCACTGATTGCTTTGATCTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGA GA
IR (inverted repeat)[243-255] [257-269] nt  (TATTTAATAATTC..GAATTATTAAATA)
[311-318] [321-328] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [337-338] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:243-255;R:257-269;L:311-318;R:321-328;D:337-338
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 775
Name TraI_pCI5 insolico
GenBank accession number WP_046201752
Family MOBF
Length 1756 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 2.80E-74, Aligned region: 10..284
TraI [PF07057.10], Evalue: 4.30E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.60E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAGNYYTNKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVGFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYRDAADQRAD
LRTLTPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLQEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAVSVKAGEESVAQ
VSGVREQAILTQTIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVSSVSEEAMTVVVPGRAEPATLPVSDSPFTALKLENGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSQVDLLTEAKSFAAEGTS
FTELGREIDAQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREVVAEGVTLYTPDKIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSYGQQT
RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK
QHVQVYTDDRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DSRTEQSVREIAGQERERAVITEREAALPESVLREPQREREAVREVARENLLQERLQQME
RDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1163
Plasmid name pCI5
GenBank accession number NZ_CP011019.1
Incompatibility group IncFIB(AP001918)
Genome size 207265 bp
Coordinate of oriT  [Strand] 136703..137244 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli CI5 [562]
Reference
[1] Mehershahi KS et al (2015) Complete Genome Sequence of Uropathogenic Escherichia coli Strain CI5. Genome Announc. 3(3). [PMID:26021932]

Microbial Bioinformatics Group in MML, SJTU