oriTDB

The information of the oriT region

oriTDB accession number	100691
Name	oriT_pECC-1470_100
Sequence Completeness	intact
oriT length	462 nt
oriT sequence	AAGAAGAGAACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGC TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAAC AAGGGTCGTCATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA ATTAAAATACATTATCTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTT GTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTT GTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGG ATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGAT CTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)	[142-151] [154-163] nt (ATAGGGTCGT..ACGACCCTAT) [231-238] [241-248] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[257-258] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:142-151;R:154-163;L:231-238;R:241-248;D:257-258

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AAGAAGAGAACCCCTAGCAGCGCCCCTAGGGGCATACTATAAAAAAACGCACGGCGCTGC

61

TAGGGGCGGCCCTAATATATCCAAATGTTTTTCATGAAAATTGTCAGCACTGACCCTAAC

121

AAGGGTCGTCATAGGGTCGCTATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAA

181

ATTAAAATACATTATCTAGAACATAAACTAATGATTTAAAAAACAAATCAGCAAAAACTT

241

GTTTTTGCGTGGGGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTT

301

GTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGG

361

ATGTTAGCCATCTGCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGAT

421

CTTGCAGGCCGGGATTACAAAATAGATCCTGATTTACTGAGA

Reference
N/A

I. Information of Relaxase

ID	763
Name	TraI_pECC-1470_100
GenBank accession number	WP_041521561
Family	MOB_F
Length	1756 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.30E-74, Aligned region: 10..284 TraI [PF07057.10], Evalue: 1.30E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.70E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 7.40E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAGNYYTDKDNYYVLGCMGERWAGQGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDVADQRAE TRTQAPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERALAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLQEGMAFTPGNTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGRPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSNSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFMALKLENGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALSVVESKNLAFSMVDLLTEAKSFAAEGTS FVDLGREINTQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPERERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKSMLKGEA FPDVPMTLYEAIVRDYTGRTPKAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVM VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKSDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGTIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVRDIAGLERDRSAISEREAALPESVLREPQRVREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1151
Plasmid name	pECC-1470_100
GenBank accession number	NZ_CP010345.1
Incompatibility group	IncFIB(AP001918)
Genome size	100061 bp
Coordinate of oriT [Strand]	64638..65099 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli ECC-1470 [758831]

Reference

[1] Leimbach A et al (2015) Complete Genome Sequences of Escherichia coli Strains 1303 and ECC-1470 Isolated from Bovine Mastitis. Genome Announc. 3(2). [PMID:25814601]