oriTDB

The information of the oriT region

oriTDB accession number	100690
Name	oriT_pAPEC-O78-3
Sequence Completeness	intact
oriT length	83 nt
oriT sequence	GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACTATGTGGCATCAGTGCGGATT
IR (inverted repeat)	[3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC)
Location of nic site	[56-57] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:3-12;R:16-25;D:56-57

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA

61

ACTATGTGGCATCAGTGCGGATT

Reference
N/A

I. Information of Relaxase

ID	762
Name	Nuclease_pAPEC-O78-3
GenBank accession number	WP_000635819
Family	MOB_P
Length	529 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 3.10E-19, Aligned region: 49..246
Note	putative relaxase
Protein sequence [Download]	MIVKFHARGKGGGSGPVDYLLGRERNREGATVLRGNPEEIRELIDATPFSKKYTSGVLSF AEKELPPGERERVMTSFERVLMPGLEKDQYSILWVEHQDKGRLELNFVIPNMELQSGKRL QPYYDRADRPRINAWQTLVNHHYGLRDPNAPENRRTLVTPNNLPKAKQEAAEAITRGLEA LYRAGAIKTRQDVTEALTAAGFEVVRTTRSSISIADPEGGRNLRLKGALYEQSFTNGNGL REETERASRFYREHAEQRVQEARRVCRQGTELKQAENQRRYQRTYNIRETATGYAEHHRE HAAQHHRGRTVPAAEKPALAGDQRAGTVSGADRAQRHGRHFPGVLGADSVAGKQDCEKYL DHQRAGKHAGETRGKNLGGDVRQWREREISGAAGRGERGKHLDGGRQERREAGQGVNEYD GTGRAFAERLRAFATGLYAAAERVGERLRRIAEDARGYATEQRDTERACRELESAGAAIS RTSRPLESVIYHEQQIRDEQERQAQELARRWHEEQEPEARIWRGPSMGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1150
Plasmid name	pAPEC-O78-3
GenBank accession number	NZ_CP010318.1
Incompatibility group	ColRNAI
Genome size	4599 bp
Coordinate of oriT [Strand]	2047..2129 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O78-789 [562]

Reference

[1] Huja S et al (2015) Genomic avenue to avian colisepticemia. MBio. 6(1). [PMID:25587010]