oriTDB
The information of the oriT region
oriTDB accession number 100685
Name oriT_pM19-A  insolico
Sequence Completeness intact
oriT length 437 nt
oriT sequence ATATGTATACCTCTTTACTTTCTTATCGCATCCAATTTATTTCACATACGAAAAAATGAC AATTCATCGATGAATCGAATCGTGATTTAACTTACGAACATTACGATTCATATGGCAAAT CACATCACGAATTGAACCTAGAGTCAATTCACTTTAACTCATTGTTTTATTATTACCGAT TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACTAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG TAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGTGCTGCTT ATTAGTTGAGTCTGTTTGTGGGGTGGGTTAAATTATTACCGTGGAACGGCACCAGAGGTG TAAGAATGAGAAAATGG
IR (inverted repeat)IR1: [201-215] [222-236] nt  (ATTCATTGGTGAATC..GATTCACTAATGAAT)
IR2: [308-315] [318-325] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [334-335] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:201-215;R:222-236;L:308-315;R:318-325;D:334-335
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 757
Name TraI_pM19-A insolico
GenBank accession number WP_073521418
Family MOBF
Length 1756 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.10E-75, Aligned region: 10..284
TraI [PF07057.10], Evalue: 1.00E-55, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.50E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGNVDKDVFTRLLEGRL
PDGSDLSRMQDGRNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDAAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFSENVLANRIAFGKIYQSDLRQRVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT
IREVVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYREAADRRAE
TRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG
IDEAINREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDTGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQTFLTQAIRRELKTQGVLGQTEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDALGETQVVRISSLDSSWSLFRPEKVPVADGERLRV
TGKIPGLRVSGGDRLQVSSVSEGTMTVIVPGRAEPASLPVSDSPFTALKLENGWVETPGH
SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTG
FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK
QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGYSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQEQDRASSSERETALPESVLREPQREREAVREVARENLLQERLQQME
REMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1145
Plasmid name pM19-A
GenBank accession number NZ_CP010222.1
Incompatibility group IncFIB(AP001918)
Genome size 146552 bp
Coordinate of oriT  [Strand] 18762..19198 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli M19 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU