oriTDB

The information of the oriT region

oriTDB accession number	100679
Name	oriT_pM9-A
Sequence Completeness	intact
oriT length	437 nt
oriT sequence	ATATGTATACCTCTTTACTTTCTTATCGCATCCAATTTATTTCACATACGAAAAAATGAC AATTCATCGATGAATCGAATCGTGATTTAACTTACGAACATTACGATTCATATGGCAAAT CACATCACGAATTGAACCTAGAGTCAATTCACTTTAACTCATTGTTTTATTATTACCGAT TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACTAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG TAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGTGCTGCTT ATTAGTTGAGTCTGTTTGTGGGGTGGGTTAAATTATTACCGTGGAACGGCACCAGAGGTG TAAGAATGAGAAAATGG
IR (inverted repeat)	IR1: [201-215] [222-236] nt (ATTCATTGGTGAATC..GATTCACTAATGAAT) IR2: [308-315] [318-325] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[334-335] nt
Conserved sequence flanking the nic site	GTAGTGTGT\|GG
Note	predicted by the oriTfinder
	L:201-215;R:222-236;L:308-315;R:318-325;D:334-335

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ATATGTATACCTCTTTACTTTCTTATCGCATCCAATTTATTTCACATACGAAAAAATGAC

61

AATTCATCGATGAATCGAATCGTGATTTAACTTACGAACATTACGATTCATATGGCAAAT

121

CACATCACGAATTGAACCTAGAGTCAATTCACTTTAACTCATTGTTTTATTATTACCGAT

181

TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACTAATGAATCCTT

241

TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAATAG

301

TAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGTGCTGCTT

361

ATTAGTTGAGTCTGTTTGTGGGGTGGGTTAAATTATTACCGTGGAACGGCACCAGAGGTG

421

TAAGAATGAGAAAATGG

Reference
N/A

I. Information of Relaxase

ID	751
Name	TraI_pM9-A
GenBank accession number	WP_073519935
Family	MOB_F
Length	1756 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.10E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 1.00E-55, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.60E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 9.70E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MLSFSVVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGNVDKDVFTRLLEGRL PDGSDLSRMQDGRNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDAAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQDPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFSENVLANRIAFGKIYQSDLRQRVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT IREVVGEDASLKSRDVAALDTRKSKQHVDPEIKMAEWMQTLKETGFDIRAYREAADRRAE TRTQTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAINREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDTGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQTFLTQAIRRELKTQGVLGQTEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDALGETQVVRISSLDSSWSLFRPEKVPVADGERLRV TGKIPGLRVSGGDRLQVSSVSEGTMTVIVPGRAEPASLPVSDSPFTALKLESGWVETPGH SVSDSAKVFASVTQMAIDNATLNGLARSGRDVWLYSSLDEARTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTS FTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGQEQVM VPVLNTANIRDGELRRLSTWENNPDALALVDSVYHRIAGISRDDGLITLEDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVFGDSVTLSDGQQT RVIRPGQERAEQHIDLAYTITAHGAQGASETFAIALEGTEGNRKQLAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGYSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSEDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQEQDRASSSERETALPESVLREPQREREAVREVARENLLQERLQQME REMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1139
Plasmid name	pM9-A
GenBank accession number	NZ_CP010197.1
Incompatibility group	IncFIB(AP001918)
Genome size	146524 bp
Coordinate of oriT [Strand]	18762..19198 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli M9 [562]

Reference
N/A