oriTDB
The information of the oriT region
oriTDB accession number 100675
Name oriT_pH8-B  insolico
Sequence Completeness intact
oriT length 550 nt
oriT sequence TTACTCTGGCCATAAGATAAAACCTTTCATTATTAAGCAATGAACTTTTCACTATAAATA TGCATATAGTGTTTACAAGTAAGAAAGACACTCCTAGCAGCGCCTCTAGGATCATCCTAT AAAAAAATGCGATCCGGCGCTAGGGGCGTCCCTAATATATATCAATGTTTTTCATGAAAA TTGTCAGTACTGATCCTAATAAGAGTCGCTATAGGGTCGTAACAGGATCGCCAACGACTC TCTATTTAATAATTCAGAATTATTAAATATAAATAGCGTTTGTTAATTACATGATTTAAA ACGTAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCA CCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAG GTGGAAAAATGAAAAAATGGATGTTAGCAATCTGCCTGATGTTTATAAATGGGATCTGCG AAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGACCCGGATTTACTGA GAGCGATATC
IR (inverted repeat)[243-255] [257-269] nt  (TATTTAATAATTC..GAATTATTAAATA)
[311-318] [321-328] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [337-338] nt
Conserved sequence flanking the
  nic site 		GTAGTGTGT|GG
Note predicted by the oriTfinder
L:243-255;R:257-269;L:311-318;R:321-328;D:337-338
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 747
Name Relaxase_pH8-B insolico
GenBank accession number WP_000130000
Family MOBC
Length 101 aa
UniProt ID _
PDB ID _
Pfam PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85
HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94
Note putative relaxase
Protein sequence [Download] MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS
RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1135
Plasmid name pH8-B
GenBank accession number NZ_CP010174.1
Incompatibility group IncFII
Genome size 106274 bp
Coordinate of oriT  [Strand] 63245..63794 [-]
Drug resistance insolico tunicamycin resistance
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli H8 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU