oriTDB
The information of the oriT region
oriTDB accession number 100667
Name oriT_pC8-A  insolico
Sequence Completeness intact
oriT length 449 nt
oriT sequence CTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCT AATATAAAACAATGTTTTTTATAAAAATAGTCAGTAGCACCCCTACAAAGCGGTGCAGAC ACGGTGCCATAGCCGCGCCTGCACCGCTGTTTTAAATAAATCTGATTTAAACAATATAAA TTCATGAATATAAGCATATGATATTAAAATCAAATCAGCAAAAACTTGCTTTTGCGTGGG GTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTA AATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCT GCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGG ATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)[151-157] [165-171] nt  (TTTAAAT..ATTTAAA)
[218-223] [230-235] nt  (GCAAAA..TTTTGC)
Location of nic site [244-245] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:151-157;R:165-171;L:218-223;R:230-235;D:244-245
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 739
Name TraI_pC8-A insolico
GenBank accession number WP_077899824
Family MOBF
Length 1756 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 7.60E-74, Aligned region: 10..284
TraI [PF07057.10], Evalue: 3.00E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 3.90E-34, Aligned region: 968..1159
AAA_19 [PF13245.5], Evalue: 9.00E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MMSIAQVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLMEGRL
PDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHSHVVVANVTQYNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE
TRTQAPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG
IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTAGYS
DAVSVLAQDRPSLAIVSGHGGAAGQREWVAELAMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR
TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQAMLTQAIRSELKTQGVLGHPEVTMTALSQVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGH
SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTS
FTELGGEINVQIKRGDLLYVDVAKGYGTGLLISRASYEAEKSILRHILEGKEAVTPLMER
VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMVPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTVFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YNLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVTLYTPDKIRVGTSDRMRFTKSDRERGYVANSVWTVTAVFGDSVTLSDGQQT
RVIRPGQEHAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK
QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKDDREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRVAITEREAALPESVLREPQREREAVREVARENLLQERLQQME
RDMVRDLQKEKTPGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1127
Plasmid name pC8-A
GenBank accession number NZ_CP010126.1
Incompatibility group IncFIB(AP001918)
Genome size 143617 bp
Coordinate of oriT  [Strand] 88465..88913 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli C8 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU