oriTDB

The information of the oriT region

oriTDB accession number	100667
Name	oriT_pC8-A
Sequence Completeness	intact
oriT length	449 nt
oriT sequence	CTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCT AATATAAAACAATGTTTTTTATAAAAATAGTCAGTAGCACCCCTACAAAGCGGTGCAGAC ACGGTGCCATAGCCGCGCCTGCACCGCTGTTTTAAATAAATCTGATTTAAACAATATAAA TTCATGAATATAAGCATATGATATTAAAATCAAATCAGCAAAAACTTGCTTTTGCGTGGG GTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTA AATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCT GCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGG ATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)	[151-157] [165-171] nt (TTTAAAT..ATTTAAA) [218-223] [230-235] nt (GCAAAA..TTTTGC)
Location of nic site	[244-245] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:151-157;R:165-171;L:218-223;R:230-235;D:244-245

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTAGCAGCGCCCCTAGCGGTATCCTATAAAAAAACACACCGCGCCGCTAGCAGCACCCCT

61

AATATAAAACAATGTTTTTTATAAAAATAGTCAGTAGCACCCCTACAAAGCGGTGCAGAC

121

ACGGTGCCATAGCCGCGCCTGCACCGCTGTTTTAAATAAATCTGATTTAAACAATATAAA

181

TTCATGAATATAAGCATATGATATTAAAATCAAATCAGCAAAAACTTGCTTTTGCGTGGG

241

GTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTTA

301

AATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATCT

361

GCCTGATGTTTATAAATGAGATCTGCCATGCCACTGATTGCTTTGATCTTGCAGGCCGGG

421

ATTACAAAATAGATCCTGATTTACTGAGA

Reference
N/A

I. Information of Relaxase

ID	739
Name	TraI_pC8-A
GenBank accession number	WP_077899824
Family	MOB_F
Length	1756 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 7.60E-74, Aligned region: 10..284 TraI [PF07057.10], Evalue: 3.00E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 3.90E-34, Aligned region: 968..1159 AAA_19 [PF13245.5], Evalue: 9.00E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVKSAGSAGNYYTDKDNYYVLGSMGERWAGKGAEQLGLQGSVDKDVFTRLMEGRL PDGADLSRMQDGRNRHRPGYDLTFSAPKSVSMMAMPGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHSHVVVANVTQYNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE TRTQAPGPASPDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSSDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGHGGAAGQREWVAELAMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQAMLTQAIRSELKTQGVLGHPEVTMTALSQVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPASLPVSDSPFTALKLENGWVETPGH SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETLLETAISLQKAGLHTPAQQAIHLALPVLESKNLAFSMVDLLTEAKSFAAEGTS FTELGGEINVQIKRGDLLYVDVAKGYGTGLLISRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMVPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTVFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YNLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDKIRVGTSDRMRFTKSDRERGYVANSVWTVTAVFGDSVTLSDGQQT RVIRPGQEHAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKDDREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQERDRVAITEREAALPESVLREPQREREAVREVARENLLQERLQQME RDMVRDLQKEKTPGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1127
Plasmid name	pC8-A
GenBank accession number	NZ_CP010126.1
Incompatibility group	IncFIB(AP001918)
Genome size	143617 bp
Coordinate of oriT [Strand]	88465..88913 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli C8 [562]

Reference
N/A