oriTDB

The information of the oriT region

oriTDB accession number	100666
Name	oriT_pC5-A
Sequence Completeness	intact
oriT length	504 nt
oriT sequence	TTTTACTCTAAATACGCATAGAGTATTCATAAGAAAGAAGAACGCTCCTAGCGGCGCTCC TAGGATCATCCTATAAAAAATTGCGATCCGCCGCTAGAGGCGGCACTAATATATCACAAT GTTTTTCATAAAAAATGTCAGTACTGATCCTAATCAGAGTCGTCACAGGATCACCATAGG GTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAGAACATAA GCTAATGATTCAGAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTT GGTGGTGAGAACCACCAACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGA TAATGTCACCAGAGGTGAAAAAATGAAAAAGTGGATGTTAGCAATCTGCCTGATGTTTAT AAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGA CCCGGATTTACTGAGAGCGATATC
IR (inverted repeat)	[176-185] [188-197] nt (ATAGGGTCGT..ACGCCCCTAT) [265-272] [275-282] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[291-292] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:176-185;R:188-197;L:265-272;R:275-282;D:291-292

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTTTACTCTAAATACGCATAGAGTATTCATAAGAAAGAAGAACGCTCCTAGCGGCGCTCC

61

TAGGATCATCCTATAAAAAATTGCGATCCGCCGCTAGAGGCGGCACTAATATATCACAAT

121

GTTTTTCATAAAAAATGTCAGTACTGATCCTAATCAGAGTCGTCACAGGATCACCATAGG

181

GTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAGAACATAA

241

GCTAATGATTCAGAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTT

301

GGTGGTGAGAACCACCAACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGA

361

TAATGTCACCAGAGGTGAAAAAATGAAAAAGTGGATGTTAGCAATCTGCCTGATGTTTAT

421

AAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGA

481

CCCGGATTTACTGAGAGCGATATC

Reference
N/A

I. Information of Relaxase

ID	738
Name	TraI_pC5-A
GenBank accession number	WP_073521273
Family	MOB_F
Length	1756 aa
UniProt ID	A0A1L4J5E1
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.20E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 4.30E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 1.30E-34, Aligned region: 968..1160 AAA_19 [PF13245.5], Evalue: 8.00E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMTEWMQTLKETGFDIRAYRDAAEQRAE TRTQAPGAVSLEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTIGILPPEEGVIERARAG IDEAISREQLIPLDREKGLFTSWIHVLDELSVRALSRDIMKQNRVTVHPEKSVSRTAGYS DAVSVLAQDRPPLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDSGQR TGTGSALMAMKDAGVNIYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ VSGVREQVILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVGDSLFTALKLESGWVETPGH SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTS FADLGREINAQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMEKLTSGQRAATRMILETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK QHVQVYTDDRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1126
Plasmid name	pC5-A
GenBank accession number	NZ_CP010123.1
Incompatibility group	IncFIB(AP001918)
Genome size	214445 bp
Coordinate of oriT [Strand]	63390..63893 [+]
Drug resistance	_
Heavy-metal resistance	copper resistance
Virulence factor	virulence-associated protein VagC
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli C5 [562]

Reference
N/A