oriTDB
The information of the oriT region
oriTDB accession number 100666
Name oriT_pC5-A  insolico
Sequence Completeness intact
oriT length 504 nt
oriT sequence TTTTACTCTAAATACGCATAGAGTATTCATAAGAAAGAAGAACGCTCCTAGCGGCGCTCC TAGGATCATCCTATAAAAAATTGCGATCCGCCGCTAGAGGCGGCACTAATATATCACAAT GTTTTTCATAAAAAATGTCAGTACTGATCCTAATCAGAGTCGTCACAGGATCACCATAGG GTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACATTATTTAGAACATAA GCTAATGATTCAGAAAACAAATCAGCAAAAACTTGTTTTTGCGTGGGGTGTGGTGCTTTT GGTGGTGAGAACCACCAACTAGTTGAGCCTTATTGTGGAGTAGGTTAAATTATTTCCGGA TAATGTCACCAGAGGTGAAAAAATGAAAAAGTGGATGTTAGCAATCTGCCTGATGTTTAT AAATGGGATCTGCGAAGCCGCCGATTGCTTTGATCTTGCAGGTCGGGATTACAAAATAGA CCCGGATTTACTGAGAGCGATATC
IR (inverted repeat)[176-185] [188-197] nt  (ATAGGGTCGT..ACGCCCCTAT)
[265-272] [275-282] nt  (GCAAAAAC..GTTTTTGC)
Location of nic site [291-292] nt
Conserved sequence flanking the
  nic site 		GTGGGGTGT|GG
Note predicted by the oriTfinder
L:176-185;R:188-197;L:265-272;R:275-282;D:291-292
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 738
Name TraI_pC5-A insolico
GenBank accession number WP_073521273
Family MOBF
Length 1756 aa
UniProt ID A0A1L4J5E1
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.20E-75, Aligned region: 10..284
TraI [PF07057.10], Evalue: 4.30E-56, Aligned region: 1434..1556
AAA_30 [PF13604.5], Evalue: 1.30E-34, Aligned region: 968..1160
AAA_19 [PF13245.5], Evalue: 8.00E-09, Aligned region: 973..1108
Note putative relaxase
Protein sequence [Download] MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL
PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSVMAMLGGDKRLIDAHNQAVDFAVRQVEAL
ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV
GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSSRSQA
IREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMTEWMQTLKETGFDIRAYRDAAEQRAE
TRTQAPGAVSLEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTIGILPPEEGVIERARAG
IDEAISREQLIPLDREKGLFTSWIHVLDELSVRALSRDIMKQNRVTVHPEKSVSRTAGYS
DAVSVLAQDRPPLAIVSGQGGAAGQRERVAELAMMAREQGREVQIIAADRRSQMNLKQDE
RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQILITDSGQR
TGTGSALMAMKDAGVNIYRWQGGEQRPATIISEPDRNVRYARLAGDFAASVKAGEESVAQ
VSGVREQVILTQAIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN
PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV
TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVGDSLFTALKLESGWVETPGH
SVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI
KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTS
FADLGREINAQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER
VPGELMEKLTSGQRAATRMILETPDRFTVVQGYAGVGKTTQFRAVMSAVNMLPESERPRV
VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM
ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV
YSLINRDVERALSGLESVKPSQVPRQEGAWVPEHSVTEFSHSQEAKLAEAQQKAMLKGEA
FPDIPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM
VPVLNTANIRDGELRRLSTWETHRDALALVDNVYHRIAGISKDDGLITLQDAEGNTRLIS
PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT
RVIRPGQERAEQHIDLAYAITAHGAQGASETFAIALEGTEGGRKQMAGFESAYVALSRMK
QHVQVYTDDRQGWTDAINNAVQKGTAHDVLEPKADREVMNAERLFSTARELRDVAAGRAV
LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNGLRGFSGE
GRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRPWNPGAIT
GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP
DGKTEQAVREIAGQERDRAAITEREAALPESVLREPQREREAVREVARENLLQERLQQME
RDMVRDLQKEKTLGGD
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1126
Plasmid name pC5-A
GenBank accession number NZ_CP010123.1
Incompatibility group IncFIB(AP001918)
Genome size 214445 bp
Coordinate of oriT  [Strand] 63390..63893 [+]
Drug resistance _
Heavy-metal resistance insolico copper resistance
Virulence factor insolico virulence-associated protein VagC
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli C5 [562]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU