oriTDB

The information of the oriT region

oriTDB accession number	100664
Name	oriT_pKLDS1.8701
Sequence Completeness	intact
oriT length	463 nt
oriT sequence	TTAGTGGATGAACAAATAAAATACGAGAGATTTTTTGTTCATTCATCCATGGTTTTAGAA AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGGAGGTGGCGTTTGCGTAAA GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC AACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATCTAT GGCTTTATATGGTCTATTTTGTTATAATAATTGTAACCGAATAGGGCGCAATGATTATTA CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG GCTATTTATGGACCAGAAAGAAGTATCACAAAATCAAACAAAG
IR (inverted repeat)	IR1: [138-147] [152-161] nt (CCCATTTTAT..ATAAAATGGG) IR2: [207-219] [223-235] nt (AAAAGGGGAAAGT..ACTTCCCCCTTTT) IR3: [242-255] [268-281] nt (ACATTGTAATACAA..TTGTATTACAATGT)
Location of nic site	[290-291] nt
Conserved sequence flanking the nic site	CTTG\|CA
Note	predicted by the oriTfinder
	L:138-147;R:152-161;L:207-219;R:223-235;L:242-255;R:268-281;D:290-291

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TTAGTGGATGAACAAATAAAATACGAGAGATTTTTTGTTCATTCATCCATGGTTTTAGAA

61

AAAAGAGGGACGATTTCGGAAGAAGAAAATCGTCTCTTTTTTTCTTCTTTTTGTATGACA

121

AAAAGAAAGATCTTTTGCCCATTTTATTTTTATAAAATGGGGAGGTGGCGTTTGCGTAAA

181

GCAAATCGACACAATCCAAAGGGGATAAAAGGGGAAAGTGAAACTTCCCCCTTTTCAAGC

241

AACATTGTAATACAAGAACGAAGTGCTTTGTATTACAATGTGATAGCTTGCAGTATCTAT

301

GGCTTTATATGGTCTATTTTGTTATAATAATTGTAACCGAATAGGGCGCAATGATTATTA

361

CAAAATCAATGACAAAGGGCGATTGAGGAATGAGCGCTGGGGCATTTTATCTTTGAGGAG

421

GCTATTTATGGACCAGAAAGAAGTATCACAAAATCAAACAAAG

Reference
N/A

I. Information of Relaxase

ID	736
Name	Relaxase_pKLDS1.8701
GenBank accession number	WP_052541636
Family	MOB_P
Length	410 aa
UniProt ID	A0A0D5ML92
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.50E-73, Aligned region: 9..280
Note	putative relaxase
Protein sequence [Download]	MATIAKISNGASAASALNYALGQDRPMHEKTEQWLQDHQLERPVELTNCRAVAVGGTNGI DPFIAKEQFDVVRQLHNQTKESNQVLRITQSFALDELNPKVQKDWQKANDLGVELAENLY PNHQSAVYTHLDGKNHVLHNHIIVNKVNLDTGKKLREQKGESVQRAREMNDRLASRENWH ILEPPKERQTETEKELIAKNEYSYMDDLRERINKSLQYVSVSSYKTFKECLSDNGVILSE RGKTFSYAFLDANNKQRRARETRLGSDFGRETILHELENRARQNEFRAVEQREPPITPLE RDTQQRESEIVSLEQAIEPRKSEALKRESKINRFIDTIKQFAGRLPELTQRVARKLKQTK EKILDDFERRFSKDMKNYEQEQQKILEKQANRDVQSEKKPTKDHDRGMSR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1124
Plasmid name	pKLDS1.8701
GenBank accession number	NZ_CP009908.1
Genome size	10600 bp
Coordinate of oriT [Strand]	7173..7635 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Lactobacillus helveticus KLDS1.8701 [1587]

Reference
N/A