 oriTDB accession number | 100658 |
| Name | oriT_pECO-824  |
Sequence Completeness  | intact |
| oriT length | 450 nt |
| oriT sequence | ATATGTATACCTCTTTATTTTCTTATCGCATCAAATTTATTTCACATATAAAAAAAAGAC AATTCATCGATGAATCGAATCGTGACGCAAGTTACGAAAATTACGATTCATGCAGCAAAT CACATCACGAATTGAATCTAGAGTCAATTCGCTTTAACTCGTTGTTTTATTATTACTGAT TCATCTATGAGTTGTGTTAAATTCATTGGTGAATCATATGCGATTCACCAATGAATCCTT TTTATAATGTAAAATAAATTAAAATACATTATTTAAAACATAAGTTAATGATTCAAGTAG CAAATCAGCAAAAACTTGTTTTTGCGTAGTGTGTGGTGCTTTTGGTGGTGAGAACCACCA ACCTGTTGAGCCTTTTTGTGGAGTGGGTTAAATTATTTACGGATAAAGTCACCAGAGGTG GAAAAATGAAAAAATGGATGTTAGCCATCT |
| IR (inverted repeat) | IR1: [201-215] [222-236] nt (ATTCATTGGTGAATC..GATTCACCAATGAAT) IR2: [308-315] [318-325] nt (GCAAAAAC..GTTTTTGC) |
| Location of nic site | [334-335] nt |
| Conserved sequence flanking the nic site | GTAGTGTGT|GG |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 730 |
| Name | Relaxase_pECO-824 |
| GenBank accession number | WP_000130000 |
| Family | MOBC |
| Length | 101 aa |
| UniProt ID | W9A157 |
| PDB ID | _ |
| Pfam | PadR [PF03551.13], Evalue: 4.70E-21, Aligned region: 15..85 HTH_34 [PF13601.5], Evalue: 2.60E-08, Aligned region: 27..94 |
| Note | putative relaxase |
| Protein sequence [Download] | MTDKDLYGGLIRLHILHHAAEEPVFGLGIIEELRRHGYEMSAGTVYPMLHGLEKKGYLTS RHERTGRRERRVYDITEQGRTALADAKTKVKELFGELVEGG |
Reference| ID | 1118 |
| Plasmid name | pECO-824 |
| GenBank accession number | NZ_CP009860.1 |
| Incompatibility group | IncFIB(AP001918) |
| Genome size | 121385 bp |
| Coordinate of oriT [Strand] | 101711..102160 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli ECONIH1 [562] |
Reference| [1] Conlan S et al (2014) Single-molecule sequencing to track plasmid diversity of hospital-associated carbapenemase-producing Enterobacteriaceae. Sci Transl Med. 6(254):254ra126. [PMID:25232178] |