oriTDB

The information of the oriT region

oriTDB accession number	100652
Name	oriT_pBTZ_6
Sequence Completeness	core
oriT length	39 nt
oriT sequence	CTTTACGATAGTAAAGTATAGTGTGCTATACTTTACATG
IR (inverted repeat)	[1-8] [9-16] nt (CTTTACGA..TAGTAAAG)
Location of nic site	[25-26] nt
Conserved sequence flanking the nic site	GTG\|CTA
Note	predicted by the oriTfinder
	L:1-8;R:9-16;D:25-26

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CTTTACGATAGTAAAGTATAGTGTGCTATACTTTACATG

Reference
N/A

I. Information of Relaxase

ID	724
Name	Putative relaxase of pBTZ_6
GenBank accession number	WP_051977349
Family	MOB_V
Length	408 aa
UniProt ID	_
PDB ID	_
Pfam	Mob_Pre [PF01076.18], Evalue: 1.20E-64, Aligned region: 1..196
Note	putative relaxase
Protein sequence [Download]	MSYAICRMQKFKMRDVKGIQIHNQREKESHTNHDIESERTHLNYDLHNDGHIDYLKTVKD KIEQNVETNRAIRKDAVVMCEFVVTSDREFFDVLDMVDPAQQKVFFKEAYSFLKDRYGEQ NIVHAAVHLDEKTPHMHVGMVPVTEENKLSAKQIFNRKELVSLQDDFHAHMVEKGFYLER GVSSDKKHVEMRKFKAMTAKEEINALEKEIGQCLQKKEVVNKQMEQLESRLNQLQNAVKS TNKVDKIEVKHKGGILRSKTVEMSVEDFEGLKTKAKASEAFKRENMTLHRKNEALQASNT DLHTTVKRLEKENEGLKPYKGKYERLAKLFDEMNKFYEKFIPKEVSKFHEIIGFCKRKVN ASINRFSSLRYSEKALNENEKKGYEAASKFLATQQKQQRKERGNEREL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1112
Plasmid name	pBTZ_6
GenBank accession number	NZ_CP009646.1
Genome size	4602 bp
Coordinate of oriT [Strand]	3527..3565 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Bacillus thuringiensis str. Al Hakam [412694]

Reference

[1] Johnson SL et al (2015) Complete genome sequences for 35 biothreat assay-relevant bacillus species. Genome Announc. 3(2). [PMID:25931591]