oriTDB

The information of the oriT region

oriTDB accession number	100650
Name	oriT_pCVMN1543
Sequence Completeness	intact
oriT length	551 nt
oriT sequence	TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTG ACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCA GCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTAT TGAGG
IR (inverted repeat)	IR1: [62-70] [71-79] nt (AACCCTTTC..GACAGGGTT) IR2: [143-150] [154-161] nt (TGGCCTGC..GGAGGCCA)
Location of nic site	_
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L: 62-70;R:71-79;L:143-150;R:154-161

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

TGTAAAGAGGCTGTGAAAGAATAAGAGCATCAAGATTCCAGATAGATAGAGGGAAATTTG

61

ACAAATTCCAAAGATGGGTTAGCCTAGTGACAGAACTAGATTCCAGTATTGGAATAATCA

121

GCTTTAAATTCCAGATAGATAGTTATGTGGATAGGAATTGGATAGGAATTGGGAGGGTAT

181

TGAGG

Reference
N/A

I. Information of Relaxase

ID	722
Name	TraI_pCVMN1543
GenBank accession number	WP_001326173
Family	MOB_H
Length	990 aa
UniProt ID	_
PDB ID	_
Pfam	TraI_2 [PF07514.10], Evalue: 6.50E-102, Aligned region: 44..362
Note	putative relaxase
Protein sequence [Download]	MLKALNKLFGGRSGVIETAPSVRVLPLKDVEDEEIPRYPPFAKGLPVAPLDKILATQAEL IEKVRNSLGFTVDDFNRLVLPVIQRYAAFVHLLPASESHHHRGAGGLFRHGLEVAFWAAQ ASESVIFSIEGTPRERRDNEPRWRLASCFSGLLHDVGKPLSDVSITDKDGSITWNPYSES LHDWAHRHEIDRYFIRWRDKRHKRHEQFSLLAVDRIIPAETREFLSKSGPSIMEAMLEAI SGTSVNQPVTKLMLRADQESVSRDLRQSRLDVDEFSYGVPVERYVFDAIRRLVKTGKWKV NEPGAKVWHLNQGVFIAWKQLGDLYDLISHDKIPGIPRDPDTLADILIERGFAVPNTVQE KGERAYYRYWEVLPEMLQEAAGSVKILMLRLESNDLVFTTEPPAAVAAEVVGDVEDAEIE FVDPEEVDDDQEEDVSALNDDMLAAEQEAEKALAGLGFGDAMEMLKSTSDAVEEKPEQKD AGSTESSKPDAGKKGKPQSKPGKAKPKSDTEKQPHKPEAKEDLSPQDIAKNAPPLANDNP LQALKDVGGGLGDIDFPFDAFSASAETASTDATNSEIPDVAMPGKQEKQPKQDFVPQEQN SLQGDDFPMFGSSDEPPSWAIEPLPMLTDAPEQTTPAPAMPPTDKPNLHEKDAKTLLVEM LAGYGEASALLEQAIMPVLEGKTTLGEVLCLMKGQAVILYPDGARSLGAPSEVLSKLSHA NAIVPDPIMPGRKVRDFSGVKAIVLAEQLSDAVVAAIKDAEASMGGYQDAFELVSPPGLD ASKNKSAPKQQSRKKAQQQKPEVNAGKPSPEQKAKGKDSQPQQKEKKVDVTSPVEEPQRQ PVQEKQNVARLPKREVQPVAPEPKVEREKELGHVEVREREEPEVREFEPPKAKTNPKDIN AEDFLPSGVTPQKALQMLKDMIQKRSGRWLVTPVLEEDGCLVTSDKAFDMIAGENIGISK HILCGMLSRAQRRPLLKKRQGKLYLEVNET

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1110
Plasmid name	pCVMN1543
GenBank accession number	NZ_CP009570.1
Incompatibility group	IncA/C2
Genome size	118585 bp
Coordinate of oriT [Strand]	21401..21951 [-]
Drug resistance	resistance to cetylpyridinium; quaternary ammonium compound-resistance
Heavy-metal resistance	mercury resistance
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Newport str. CVM N1543 [858307]

Reference

[1] Cao G et al (2015) Complete Sequences of Six IncA/C Plasmids of Multidrug-Resistant Salmonella enterica subsp enterica Serotype Newport. Genome Announc. 3(1). [PMID:25720681]