oriTDB
The information of the oriT region
oriTDB accession number 100648
Name oriT_pLA2  insolico
Sequence Completeness intact
oriT length 190 nt
oriT sequence GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA CATTAGCCGCCGCAAGGCTTATCCTGCCATACAGTAGATACCTACCCCACAAACAAAAAG CGGTGGCAACTGCCACCGCTCTAAATTCCACGTCGCCTCGATGCTGAGAGAACGTGGACG GTAAAAGCTA
IR (inverted repeat)IR1: [19-38] [39-58] nt  (AACCGTGAAGCGCGATCCGA..TAGGATTGAGCGTATCGGTT)
IR2: [117-128] [131-142] nt  (AAAGCGGTGGCA..TGCCACCGCTCT)
Location of nic site [86-87] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note predicted by the oriTfinder
L:19-38;R:39-58;L:117-128;R:131-142;D:86-87
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 720
Name Putative relaxase of pLA2 insolico
GenBank accession number WP_004213271
Family MOBP
Length 347 aa
UniProt ID G6ELI0
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.80E-06, Aligned region: 72..260
Note putative relaxase
Protein sequence [Download] MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARVVSRAP
EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEARAELARDWGDPVYWRD
NSTVAAVSMVFSMPAGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR
GDTGQRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA
GTAPAPFANQKITAAARDQLAGRSTAPDFVVRGRQAWNETQHRYLAAAKRLETSSDPADR
QLADQVRQFVGAGRTPTIHERSVAAMERKRQSERSRNRDRSREGPGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1108
Plasmid name pLA2
GenBank accession number NZ_CP009296.1
Genome size 62341 bp
Coordinate of oriT  [Strand] 1027..1216 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Novosphingobium pentaromativorans US6-1 [1088721]
Reference
[1] Choi DH et al (2015) Complete genome sequence of Novosphingobium pentaromativorans US6-1(T). Stand Genomic Sci. 0.490972222. [PMID:26594308]

Microbial Bioinformatics Group in MML, SJTU