oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100635
Name	oriT_pOV14c
Sequence Completeness	core
oriT length	44 nt
oriT sequence	GGATCCAAGGGCGCAATTATACGTCGCTGATGCGACGTGCTTGC
IR (inverted repeat)	[21-27] [32-38] nt (ACGTCGC..GCGACGT)
Location of nic site	[11-12] nt
Conserved sequence flanking the nic site	_
Note	predicted by the oriTfinder
	L:21-27;R:32-38;D:11-12

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	707
Name	TraA_pOV14c
GenBank accession number	WP_025430013
Family	MOB_Q
Length	1100 aa
UniProt ID	W8ID92
PDB ID	_
Pfam	MobA_MobL [PF03389.14], Evalue: 1.70E-77, Aligned region: 17..243 AAA_30 [PF13604.5], Evalue: 5.30E-56, Aligned region: 381..569 AAA_19 [PF13245.5], Evalue: 3.10E-10, Aligned region: 386..513 Viral_helicase1 [PF01443.17], Evalue: 8.10E-05, Aligned region: 657..737 UvrD_C_2 [PF13538.5], Evalue: 1.90E-06, Aligned region: 692..736
Note	putative relaxase
Protein sequence [Download]	MAIAHFSASIVSRGGGRSVVLSAAYRHCAKMEYEREARTIDYTRKEGLLHEEFMLPADAP KWVRAMIADRSVAGASEAFWNKVEAFEKRSDAQLARDLTIALPLELTPEQNIALVRDFVE RHIHAKGMVADWVYHDNPGNPHIHLMTTLRPLTEEGFGSKKVAVNGEDGQPVRTKSGKIL YELWAGSTDDFNSLRDGWFERLNHHLALGGIDLRIDGRSYEKQGIELEPTIHLGVGAKAI ERKAQEQGVRPELERIELNKERRSENARRILRNPGIVVDLITREKSVFDERDVAKVLHRY VDDPTVFQQLMLRIILNPEVLRLQRDTIEFATGEKVPARYSTRAMIRLEATMVRQALWLS NRDGHAVSDAALDVTFRRHERLSQEQKAAIERIAGPARIAAVVGRAGAGKTTMMKAAREV WELAGYRVVGGALAGKAAEGLEKEAGIQSRTLASWELRWNRGRDVLDDKTVFVMDEAGMV ASKQMADFVDAVVRAGAKIVLVGDPEQLQPIEAGAAFRAIVNRIGYAELETIYRQREDWM RKASLDLARGNVEKALTAYDANIRITGTRLKAEAVERLIGDWNHDYDQTKTTLILAHLRR DVRMLNVMAREKLVERGIVGEGHVFKTADGIRQFDAGDQIVFLKNEGLLGVKNGMIAHVT EAAPNRIVAVIGEGDQRRQVIVEQRFYSNLDHGYATTIHKSQGATVDRVKVLASLSLDRH LTYVAMTRHREDLQLYYGHRSFAFNGGLAKVLSRKNAKETTLDYERGKLYREALRYAENR GLNIVQVARTLLRDQLDWTLRQKTKLVDLGQRLAVFAERLGLHHPPKTQTMKEAAPMVAG IKTFSGSVADTVEHKLDTDPALRQQWEEVSTRFVYVFADPETAFRAMNFDAVLVDKETAK LALQTLTTAPASIGPLRGKTGILASKTEREDRRVADLNVPALKRDLEQYLRMRETAVQRL QADEQVLRQRVSIDIPALSPAAHVVLERVRDAIDRNDLPAAMAYALSNRETKLEIDGFNK AVTERFGERTLLTNAAREPSGKLFDKLSEGMQPEQKERLTEAWPIMRASQQLAAHERTVQ TLRQAEDLRLAPRQTPVMKQ

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1095
Plasmid name	pOV14c
GenBank accession number	NZ_CP007238.1
Genome size	125203 bp
Coordinate of oriT [Strand]	52599..52642 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Ensifer adhaerens OV14 [1416753]

Reference

[1] Rudder S et al (2014) Genome sequence of Ensifer adhaerens OV14 provides insights into its ability as a novel vector for the genetic transformation of plant genomes. BMC Genomics. 0.811111111. [PMID:24708309]