oriTDB

The information of the oriT region

oriTDB accession number	100632
Name	oriT_pO145-12581
Sequence Completeness	intact
oriT length	92 nt
oriT sequence	CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA TACGATTACACATCCTGTCCCGTTTTTCAGGC
IR (inverted repeat)	IR1: [7-15] [17-25] nt (GTCGGGGCT..AGCCCTGAC) IR2: [32-49] [52-69] nt (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC)
Location of nic site	[77-78] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:7-15;R:17-25;L:32-49;R:52-69;D:77-78

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA

61

TACGATTACACATCCTGTCCCGTTTTTCAGGC

Reference
N/A

I. Information of Relaxase

ID	704
Name	NikB_pO145-12581
GenBank accession number	WP_000991402
Family	MOB_P
Length	906 aa
UniProt ID	A0A023Z8L8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.20E-49, Aligned region: 61..315
Note	putative relaxase
Protein sequence [Download]	MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRL RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHY ILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI NCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF REYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDRAREGVALSSFG PLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRHF AERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL FSDKESLWGYGLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE LTPVKASSIHPDLTLSRAEPQAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDP EIRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVS QLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTR TGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWE PPSPGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1092
Plasmid name	pO145-12581
GenBank accession number	NZ_CP007138.1
Incompatibility group	IncFIB(AP001918)
Genome size	87120 bp
Coordinate of oriT [Strand]	30142..30233 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O145:H28 str. RM12581 [1248823]

Reference
N/A