oriTDB
The information of the oriT region
oriTDB accession number 100625
Name oriT_PCN061p4  insolico
Sequence Completeness intact
oriT length 354 nt
oriT sequence ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCTCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACAGATGTAATACATACAAATCT AGCATATATGCGGCTTAGAACCACATGTGTAATACGTTGCGTATTACACATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTG TATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACAC GCATAAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG
IR (inverted repeat)IR1: [145-155] [161-171] nt  (ATGTGTAATAC..GTATTACACAT)
IR2: [208-218] [222-232] nt  (GCATTTACGAT..ATCGTAATTGC)
IR3: [335-343] [346-354] nt  (CTTTCATGC..GCATGAAAG)
Location of nic site [241-242] nt
Conserved sequence flanking the
  nic site 		GGTGT|ATAGC
Note predicted by the oriTfinder
L:145-155;R:161-171;L:208-218;R:222-232;L:335-343;R:346-354;D:241-242
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 697
Name Relaxase_PCN061p4 insolico
GenBank accession number WP_033544595
Family MOBF
Length 968 aa
UniProt ID _
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 1.50E-80, Aligned region: 12..288
AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659
Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..654
Viral_helicase1 [PF01443.17], Evalue: 2.20E-06, Aligned region: 780..897
UvrD_C_2 [PF13538.5], Evalue: 2.10E-09, Aligned region: 849..896
Note putative relaxase
Protein sequence [Download] MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD
GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVSLQALVNGDKRIIEAHDRAVTKAIEE
AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL
TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE
AELAKNGLSRETASHELKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA
ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD
DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRSKDEARKLVD
KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ
LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG
LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK
AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH
DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQAELGLKGQGINYPLLNRLDT
TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR
FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKSVQSGEVLLENEKGRLVKID
ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE
KKLDKAASREGFKTAALELEQLKRYAKEQQHDTREKGRDKAEPTQDKGNARPKDKGHEKN
NDDRALRL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1085
Plasmid name PCN061p4
GenBank accession number NZ_CP006640.1
Genome size 34692 bp
Coordinate of oriT  [Strand] 21166..21519 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli PCN061 [1358422]
Reference
[1] Liu C et al (2015) Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033. BMC Genomics. 1.164583333. [PMID:26391348]

Microbial Bioinformatics Group in MML, SJTU