oriTDB

The information of the oriT region

oriTDB accession number	100625
Name	oriT_PCN061p4
Sequence Completeness	intact
oriT length	354 nt
oriT sequence	ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCTCTTCGGTCACTC TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACAGATGTAATACATACAAATCT AGCATATATGCGGCTTAGAACCACATGTGTAATACGTTGCGTATTACACATTAAAACACA AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTG TATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACAC GCATAAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG
IR (inverted repeat)	IR1: [145-155] [161-171] nt (ATGTGTAATAC..GTATTACACAT) IR2: [208-218] [222-232] nt (GCATTTACGAT..ATCGTAATTGC) IR3: [335-343] [346-354] nt (CTTTCATGC..GCATGAAAG)
Location of nic site	[241-242] nt
Conserved sequence flanking the nic site	GGTGT\|ATAGC
Note	predicted by the oriTfinder
	L:145-155;R:161-171;L:208-218;R:222-232;L:335-343;R:346-354;D:241-242

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

ACTCCGATACGTTGCCCTCCGAACGGTATTCAAGGTCGATTTTTTGCTCTTCGGTCACTC

61

TTAAACTGATAGATGGCATAGGTTTCCTTTGTGTAATACAGATGTAATACATACAAATCT

121

AGCATATATGCGGCTTAGAACCACATGTGTAATACGTTGCGTATTACACATTAAAACACA

181

AATTAGAATAATTTGTTTTGTTTTCAAGCATTTACGATGAAATCGTAATTGCGTATGGTG

241

TATAGCCATTAAGGGATACCATAACACGCCTTTTTTAAGGGAGAAACAGCTCTTACACAC

301

GCATAAAACGCTCAAAAAGCGTTCACATTCACCACTTTCATGCTTGCATGAAAG

Reference
N/A

I. Information of Relaxase

ID	697
Name	Relaxase_PCN061p4
GenBank accession number	WP_033544595
Family	MOB_F
Length	968 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 1.50E-80, Aligned region: 12..288 AAA_30 [PF13604.5], Evalue: 2.90E-40, Aligned region: 475..659 Viral_helicase1 [PF01443.17], Evalue: 1.10E-07, Aligned region: 495..654 Viral_helicase1 [PF01443.17], Evalue: 2.20E-06, Aligned region: 780..897 UvrD_C_2 [PF13538.5], Evalue: 2.10E-09, Aligned region: 849..896
Note	putative relaxase
Protein sequence [Download]	MLNITTITRQRVDKVVDYYADGADDYYAKDGNAMQWQGAGAEELGLTGEVEQKRFAKLLD GKVTDDISVMRKTANSESKERLGYDLTFSAPKGVSLQALVNGDKRIIEAHDRAVTKAIEE AERLALGRFTVNKKTHVENTNKLIVGKFRHETSRELDPDLHTHAFVMNLTKTSDGKWRSL TNDGIVNSLTLLGNVYKSELARELELAGYNLRYDRNGTFDLAHFSQEQIAQFSQRSQQIE AELAKNGLSRETASHELKNAAALKTRKSKGEVDRDVLYEGWKNRAKELQIDFDSREWKGA ANDDIARNTQPAALEKPIEYQADKVMTFAIRSLTERQSVITERELMDVAMKHGYGRLTLD DIRAARERAITSGNLIKEEATYAAATTNKKQQNVAMTREQWVTELVKAGRSKDEARKLVD KGITNGRLVQQKPRFTTQLAQQRERNILKMEREGRGKIQTPYTREFSEGWLASRTLKPEQ LKAVMGIIHTPNQFISVHGFAGTGKSYMTKSAADFLKEQGVHVTSLAPYGSQVKALQAEG LESRTLQSFLRASDKKIGPGSVVFIDEAGVIPARQMEETMRVIRDAGARAVFLGDTKQTK AVEAGKPFEQLIKAGMETAYMKDIQRQKDPELLKAALNAAEGKIKASLTHVTSIVEEKNH DQRYRRIVGDYVAMPPTDRANALIITGTNDSRKKINEYIQAELGLKGQGINYPLLNRLDT TQAQRQHSKYYEKGAVIIPERDYSNGLKRGAVYTVLDTGPGNKLTVSDGSGDTIAFSPAR FSKLSVYSVEKTELAVGDQVRITRNDAHLDLANGDRFKVKSVQSGEVLLENEKGRLVKID ANKPMYLGLAYASTVHSAQGLTCDKVFINMDTRSLTTAKDVYYVAVTRAKHEAVIYTDDE KKLDKAASREGFKTAALELEQLKRYAKEQQHDTREKGRDKAEPTQDKGNARPKDKGHEKN NDDRALRL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1085
Plasmid name	PCN061p4
GenBank accession number	NZ_CP006640.1
Genome size	34692 bp
Coordinate of oriT [Strand]	21166..21519 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli PCN061 [1358422]

Reference

[1] Liu C et al (2015) Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033. BMC Genomics. 1.164583333. [PMID:26391348]