oriTDB

The information of the oriT region

oriTDB accession number	100624
Name	oriT_PCN061p2
Sequence Completeness	intact
oriT length	186 nt
oriT sequence	GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT ACTGGCTCAATATGTTATGGCGGAGTAACACTTAATGAAAGTGCTTCATGTGGCAGGGGA AAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTC GCTCAC
IR (inverted repeat)	[14-21] [25-32] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[65-66] nt
Conserved sequence flanking the nic site	CTGG\|CTCA
Note	predicted by the oriTfinder
	L:14-21;R:25-32;D:65-66

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT

61

ACTGGCTCAATATGTTATGGCGGAGTAACACTTAATGAAAGTGCTTCATGTGGCAGGGGA

121

AAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTC

181

GCTCAC

Reference
N/A

I. Information of Relaxase

ID	696
Name	MbeA_PCN061p2
GenBank accession number	WP_000635824
Family	MOB_P
Length	499 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 6.90E-18, Aligned region: 41..247
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKYTSGVLSF AEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNMELLTGKRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKTKQEAAQAITRGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGKGL RAEIESAAAEYQRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERHG AYGQPDVADHRPGVCTADSIGRKHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTRLNVIEAHREQERAAQHQ KTLELERSQRQRTYNGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1084
Plasmid name	PCN061p2
GenBank accession number	NZ_CP006638.1
Genome size	5754 bp
Coordinate of oriT [Strand]	1625..1810 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli PCN061 [1358422]

Reference

[1] Liu C et al (2015) Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033. BMC Genomics. 1.164583333. [PMID:26391348]