oriTDB
The information of the oriT region
oriTDB accession number 100624
Name oriT_PCN061p2  insolico
Sequence Completeness intact
oriT length 186 nt
oriT sequence GGTTTTAGCAGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTAT ACTGGCTCAATATGTTATGGCGGAGTAACACTTAATGAAAGTGCTTCATGTGGCAGGGGA AAAATGCAGCTGCAGGGCGTACAGGAGAAAATGGAGGTTACGGTGATATGACGCCTCCTC GCTCAC
IR (inverted repeat)[14-21] [25-32] nt  (TTCGGGGC..GCCCTGAA)
Location of nic site [65-66] nt
Conserved sequence flanking the
  nic site 		CTGG|CTCA
Note predicted by the oriTfinder
L:14-21;R:25-32;D:65-66
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 696
Name MbeA_PCN061p2 insolico
GenBank accession number WP_000635824
Family MOBP
Length 499 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 6.90E-18, Aligned region: 41..247
Note putative relaxase
Protein sequence [Download] MIVKFHPRGRGGGAGPVDYLLGKDRQRDGATVLQGKPEEVRELIDASPYAKKYTSGVLSF
AEKDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHQDKGRLELNFLIPNMELLTGKRL
QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSALPKTKQEAAQAITRGLLA
LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGKGL
RAEIESAAAEYQRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERHG
AYGQPDVADHRPGVCTADSIGRKHSVVAGAADTRQLHDHPGAEEHAGHAVREEQRRAALD
LRRAETPLREGEPGSGTVRRGFELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE
RLRGMADDVWSYATGERGAERARHGLEQAGAEFERAAAPVVTRLNVIEAHREQERAAQHQ
KTLELERSQRQRTYNGPSL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1084
Plasmid name PCN061p2
GenBank accession number NZ_CP006638.1
Genome size 5754 bp
Coordinate of oriT  [Strand] 1625..1810 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli PCN061 [1358422]
Reference
[1] Liu C et al (2015) Genome analysis and in vivo virulence of porcine extraintestinal pathogenic Escherichia coli strain PCN033. BMC Genomics. 1.164583333. [PMID:26391348]

Microbial Bioinformatics Group in MML, SJTU