oriTDB

The information of the oriT region

oriTDB accession number	100623
Name	oriT_pO145-13516
Sequence Completeness	intact
oriT length	450 nt
oriT sequence	CCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGCCAGGGGCGGCCC TAACATATCCAAATGTTTTTCATGAAAATTGTCAGTACTGACCCTAACAAGGGTCGTCAT AGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACAT TATTCATAACATAAGCTAATGATTCAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGG GGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTT AAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATC TGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTGATCTTGCAGGCCGG GATTACAAAATAGATCCTGATTTACTGAGA
IR (inverted repeat)	[130-139] [142-151] nt (ATAGGGTCGT..ACGCCCCTAT) [219-226] [229-236] nt (GCAAAAAC..GTTTTTGC)
Location of nic site	[245-246] nt
Conserved sequence flanking the nic site	GTGGGGTGT\|GG
Note	predicted by the oriTfinder
	L:130-139;R:142-151;L:219-226;R:229-236;D:245-246

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

CCTAGCAGCGCCCCTAGGGTCATACTATAAAAAAACGCACGGCGCTGCCAGGGGCGGCCC

61

TAACATATCCAAATGTTTTTCATGAAAATTGTCAGTACTGACCCTAACAAGGGTCGTCAT

121

AGGGTCGCCATAGGGTCGTCAACGACCCTATTTAATAATGTAAAATAAATTAAAATACAT

181

TATTCATAACATAAGCTAATGATTCAAAAAACAAATCAGCAAAAACTTGTTTTTGCGTGG

241

GGTGTGGTGCTTTTGGTGGTGAGAACCACCAACCTGTTGAGCCTTTTTGTGGAGTGGGTT

301

AAATTATTTACGGATAAAGTCACCAGAGGTGGAAAAATGAAAAAATGGATGTTAGCCATC

361

TGCCTGATGTTTATAAATGAGATCTGCCAGGCCACTGATTGCTTTGATCTTGCAGGCCGG

421

GATTACAAAATAGATCCTGATTTACTGAGA

Reference
N/A

I. Information of Relaxase

ID	695
Name	TraI_pO145-13516
GenBank accession number	WP_025380714
Family	MOB_F
Length	1756 aa
UniProt ID	_
PDB ID	_
Pfam	TrwC [PF08751.10], Evalue: 3.40E-75, Aligned region: 10..284 TraI [PF07057.10], Evalue: 3.00E-56, Aligned region: 1434..1556 AAA_30 [PF13604.5], Evalue: 4.80E-34, Aligned region: 968..1159 AAA_19 [PF13245.5], Evalue: 7.70E-09, Aligned region: 973..1108
Note	putative relaxase
Protein sequence [Download]	MMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFTRLLEGRL PDGADLSRMQDGSNKHRPGYDLTFSAPKSVSMMAMLGGDKRLIEAHNQAVDFAVRQVEAL ASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWKTLSSDKV GKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEAFSGRSQT IREAVGEDASLKSRDVAALDTRKSKQHVDPEVRMAEWMQTLKETGFDIRAYRDAADQRAE TRTQAPGAVSQEGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGVIERARAG IDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSVPRTAGYS DAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQMNLKQDE RLSGELITGRRQLLEGMAFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVLITDSGQR TGTGSALMAMKDAGVNTYRWQGGEQRPASIISEPDRNVRYTRLAGDFAASVKAGEESVAQ VSGVREQAILTQAIRSELKTQGVLGHQEVTMTALSPVWLDSRSRYLRDMYRPGMVMEQWN PETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVADGERLRV TGKIPGLRVSGGDRLQVASVSEDAMTVVVPGRAEPATLPVADSPFTALKLENGWVETPGH SVSDSAKVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSFTVVSEQI KARAGETSLETAISHQKSALHTPAQQAIHLALPVVESKNLAFSHVDLLTEAKSFAAEGTS FTELGREIDAQIKRGDLLHVDVAKGYGTDLLVSRASYEAEKSILRHILEGKEAVTPLMER VPGELMETLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLSASERPRV VGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESSMVGNTDM ARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQTPELREAV YSLINRDVERALSGLESVKPSQVPRLEGAWAPEHSVTEFSHSQEAKLAEAQQKAMLKGEA FPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDAREKAGELGKEQVM VPVLNTANIRDGELRRLSTWENNPDALALVDNVYHRIVGISKDDGLIILQDAEGNTRLIS PREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVTLSDGQQT RVIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKQMAGFESAYVALSRMK QHVQVYTDNRQGWTDAINNAVQKGTAHDVLEPKPDREVMNAQRLFSTARELRDVAAGRAV LRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGRSAGIWLNPLTTDDGNGLRGFSGE GRVKGSGDAQFVSLQGSRNGESLLADNMQEGVRIARDNPDSGVVVRIAGEGRPWNPGAIT GGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMAENKPDLP DGRTEQAVREIAGQERDRAAITEREAALPESVLRESQREREAVREVARENLLQERLQQME RDMVRDLQKEKTLGGD

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1083
Plasmid name	pO145-13516
GenBank accession number	NZ_CP006263.1
Incompatibility group	IncFIB(AP001918)
Genome size	98066 bp
Coordinate of oriT [Strand]	58312..58761 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli O145:H28 str. RM13516 [1248915]

Reference

[1] Cooper KK et al (2014) Comparative genomics of enterohemorrhagic Escherichia coli O145:H28 demonstrates a common evolutionary lineage with Escherichia coli O157:H7. BMC Genomics. 15:17. [PMID:24410921]