oriTDB
The information of the oriT region
oriTDB accession number 100621
Name oriT_pO145-13514  insolico
Sequence Completeness intact
oriT length 92 nt
oriT sequence CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA TACGATTACACATCCTGTCCCGTTTTTCAGGC
IR (inverted repeat)IR1: [7-15] [17-25] nt  (GTCGGGGCT..AGCCCTGAC)
IR2: [32-49] [52-69] nt  (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC)
Location of nic site [77-78] nt
Conserved sequence flanking the
  nic site 		CATCCTG|T
Note predicted by the oriTfinder
L:7-15;R:17-25;L:32-49;R:52-69;D:77-78
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 693
Name NikB_pO145-13514 insolico
GenBank accession number WP_000991402
Family MOBP
Length 906 aa
UniProt ID _
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.20E-49, Aligned region: 61..315
Note putative relaxase
Protein sequence [Download] MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEMKAEIPHRNRFRRLVDYATRL
RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHY
ILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI
NCLPWSQEKLSRACRELEIKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF
REYIVQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDRAREGVALSSFG
PLWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRHF
AERLPEMARNGELESCLDVHRTLATAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT
LDYMQDLDGGWQPVPKDIFTQVIPGERFRGRNLGTQAVSDYEWYRMRMGTGPQGAIKREL
FSDKESLWGYGLIQCRSLIEEMIADGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE
LTPVKASSIHPDLTLSRAEPQAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDP
EIRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP
QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVS
QLRGWDYRDRRSRNKDKRRTTNVDRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTR
TGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM
VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSVSHDDNYTLRQEKTWE
PPSPGM
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1081
Plasmid name pO145-13514
GenBank accession number NZ_CP006028.1
Incompatibility group IncFIB(AP001918)
Genome size 87120 bp
Coordinate of oriT  [Strand] 30142..30233 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Escherichia coli O145:H28 str. RM13514 [1248902]
Reference
[1] Cooper KK et al (2014) Comparative genomics of enterohemorrhagic Escherichia coli O145:H28 demonstrates a common evolutionary lineage with Escherichia coli O157:H7. BMC Genomics. 15:17. [PMID:24410921]

Microbial Bioinformatics Group in MML, SJTU