oriTDB
The information of the oriT region
oriTDB accession number 100618
Name oriT_pTKS  insolico
Sequence Completeness intact
oriT length 191 nt
oriT sequence GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA CATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAG CGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGAC GGTAAAAGCTA
IR (inverted repeat)IR1: [19-38] [39-58] nt  (AACCGTGAAGCGCGATCCGA..TAGGATTGAGCGTATCGGTT)
IR2: [119-128] [131-140] nt  (AGCGGTGGCA..TGCCACCGCT)
Location of nic site [86-87] nt
Conserved sequence flanking the
  nic site 		TATCCTG|C
Note predicted by the oriTfinder
L:19-38;R:39-58;L:119-128;R:131-140;D:86-87
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 690
Name Putative relaxase of pTKS insolico
GenBank accession number WP_021245546
Family MOBP
Length 347 aa
UniProt ID A0A126RXL4
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 1.90E-07, Aligned region: 72..261
Note putative relaxase
Protein sequence [Download] MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARIVSRAP
EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAALARDWGDPVYWRD
NSTVAAVSMVFSMPAGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR
GDTGKRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA
GTAPAPFANQKITAAARDQLAGRSTAPDFVVRGRQAWTETQHRYLAAAKRLEASSDPADR
QLADQVRQFVGAGRTPTIHERSVAAMERKRQSERSRNRDRSREGPGR
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1078
Plasmid name pTKS
GenBank accession number NZ_CP005089.1
Genome size 53908 bp
Coordinate of oriT  [Strand] 26685..26875 [-]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Sphingobium sp. TKS [1315974]
Reference
[1] Tabata M et al (2016) Complete Genome Sequence of a γ-Hexachlorocyclohexane Degrader, Sphingobium sp Strain TKS, Isolated from a γ-Hexachlorocyclohexane-Degrading Microbial Community. Genome Announc. 4(2). [PMID:27056231]

Microbial Bioinformatics Group in MML, SJTU