oriTDB

The information of the oriT region

oriTDB accession number	100618
Name	oriT_pTKS
Sequence Completeness	intact
oriT length	191 nt
oriT sequence	GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA CATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAG CGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGAC GGTAAAAGCTA
IR (inverted repeat)	IR1: [19-38] [39-58] nt (AACCGTGAAGCGCGATCCGA..TAGGATTGAGCGTATCGGTT) IR2: [119-128] [131-140] nt (AGCGGTGGCA..TGCCACCGCT)
Location of nic site	[86-87] nt
Conserved sequence flanking the nic site	TATCCTG\|C
Note	predicted by the oriTfinder
	L:19-38;R:39-58;L:119-128;R:131-140;D:86-87

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GTGGGGTAGGTATCTCCCAACCGTGAAGCGCGATCCGATAGGATTGAGCGTATCGGTTAA

61

CATTAGCCGCCGCAAGGCTTATCCTGCCATAAAGTAGATACCTACCCCACAAACAAAAAG

121

CGGTGGCAACTGCCACCGCTTCAAAATTCCACGCCGCCTCAATGCTGAGTGAACGTGGAC

181

GGTAAAAGCTA

Reference
N/A

I. Information of Relaxase

ID	690
Name	Putative relaxase of pTKS
GenBank accession number	WP_021245546
Family	MOB_P
Length	347 aa
UniProt ID	A0A126RXL4
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 1.90E-07, Aligned region: 72..261
Note	putative relaxase
Protein sequence [Download]	MSDFDSSFEAGQLAALFKPKLTSDPNRRGADMLLRSLSSRRAGQGGSTRARLARIVSRAP EVMVKVTGRPKGKNHAAAHFDYIGRKGDVPLETRDGDILTDKEDRAALARDWGDPVYWRD NSTVAAVSMVFSMPAGTDPDKVLSAVREVARSEIGHEWDYVLALHTDTPRPHVHVTVAAR GDTGKRFNPRPQTLHHYRERFAEELRARGVTAEATPRAARGVGRAGQSMALNRMRQRYMA GTAPAPFANQKITAAARDQLAGRSTAPDFVVRGRQAWTETQHRYLAAAKRLEASSDPADR QLADQVRQFVGAGRTPTIHERSVAAMERKRQSERSRNRDRSREGPGR

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1078
Plasmid name	pTKS
GenBank accession number	NZ_CP005089.1
Genome size	53908 bp
Coordinate of oriT [Strand]	26685..26875 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Sphingobium sp. TKS [1315974]

Reference

[1] Tabata M et al (2016) Complete Genome Sequence of a γ-Hexachlorocyclohexane Degrader, Sphingobium sp Strain TKS, Isolated from a γ-Hexachlorocyclohexane-Degrading Microbial Community. Genome Announc. 4(2). [PMID:27056231]