oriTDB

The information of the oriT region

oriTDB accession number	100616
Name	oriT_pKP13b
Sequence Completeness	intact
oriT length	193 nt
oriT sequence	GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCG GAGTGTATACTGGCTAAACATGTTATGACGGAGTGGCAATTTATGAAAGTGCTTCATGTG ACAGGGGAAAAATGCGGCTGCCGGGCGTAAGTGCAAAATGCAGGTGGTGATGAAATGACG CCTCCTCGCTCAC
IR (inverted repeat)	[22-29] [33-40] nt (TTCGGGGC..GCCCTGAA)
Location of nic site	[73-74] nt
Conserved sequence flanking the nic site	CTGG\|CTAA
Note	predicted by the oriTfinder
	L:22-29;R:33-40;D:73-74

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GCGGCAGTGGTTTTAGCGGGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCG

61

GAGTGTATACTGGCTAAACATGTTATGACGGAGTGGCAATTTATGAAAGTGCTTCATGTG

121

ACAGGGGAAAAATGCGGCTGCCGGGCGTAAGTGCAAAATGCAGGTGGTGATGAAATGACG

181

CCTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	688
Name	Putative relaxase of pKP13b
GenBank accession number	WP_045265544
Family	MOB_P
Length	497 aa
UniProt ID	_
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 2.80E-17, Aligned region: 42..273
Note	putative relaxase
Protein sequence [Download]	MIVKFHPRGRGGGGGPVDYLLGKDRQRDGASVLQGKPDEVRELIDASPYAKKYTSGVLSF AEQDLPPGQREKLMASFERVLMPGLDKDQYSVLWVEHRDKGRLELNFLIPNTELLTGKRL QPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRVLVSPSALPEAKQEAAQAITSGLLA LASSGELKTRQDVTEALESAGFEVVRTTKSSISIADPDGGRNIRLKGAIYEQSFNAGEGL RAEIESAAADYRRDAESRIQRAREVCQSGTERKREENQRRHPRPRPEAVLSHEPAHERDA AHGQPDVADHRSGLRAADSVERGHSVVAGAADTRELYDHPGAEEHAGHAVREEQRRTALD LRRAETPLREGEPGSGTVRRGLELDDTGGEIAHDGAGKTVAERIRAATAGLLEKAGRVGE RLRGMADDVWSYSTGERGAERARHGLEQAGAEFERAAAPVVERLNAIETHRQQERAVQHQ KALELERSQWQHHGPSL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1076
Plasmid name	pKP13b
GenBank accession number	NZ_CP003994.1
Genome size	3223 bp
Coordinate of oriT [Strand]	2399..2591 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Klebsiella pneumoniae subsp. pneumoniae Kp13 [1123862]

Reference

[1] Ramos PI et al (2014) Comparative analysis of the complete genome of KPC-2-producing Klebsiella pneumoniae Kp13 reveals remarkable genome plasticity and a wide repertoire of virulence and resistance mechanisms. BMC Genomics. 15:54. [PMID:24450656]