oriTDB
The information of the oriT region
oriTDB accession number 100615
Name oriT_pG6809-2  insolico
Sequence Completeness intact
oriT length 658 nt
oriT sequence AGCGCCGCAGATAATCTGACCGATTACCTCCTGAAACCAGGTCTATATAGGCCAAAAGTT CATCTGATACTTTTGCGGTTATTATTGGCATTCAGTCCTCACATTGTGCATTTCTTAAAC AAAAAATTGGGATCTAACAAGCTGAAATCTTAGTATTACCAAAGTAATAAAGCAAACTCA TTATAAATCAATGTGTTATTTGGTGTTTTTAATACCTAATTATTACCGAACATTGACGTT ATTTATTTTTTTATCTTTTAAATCAGTATGATAGCGTGATTTATCGCGCTGCGTTAGGTG TATAGCAGGTATATAGCAGGTATAGCAGGTTAAGGGATAAAAAATCATCTTTTTTGGTAG GAGCGATCTACGTAGGTTAAGGACTAACTGGCTAAAAAGCGTTCAATATTCCGTATTCAT GCTTGCATGAATACCAGTACAACACTATTACAACAAAAGTACATCAAAATTACATCAAAA TTACATCACTTGAAGGTTGACAGTACAACAGAATTACATCATTATTTGGTCATGAGGTAG CCAGTACAACAAAAGTACATCAAAGGTACATCAAAAATACATCAAAATTACATCATTCTA AATGAGGGTACTATGAAGCCCAAAAGTATCAGGGCGGCACTTCAATTGATGTTGCCGG
IR (inverted repeat)IR1: [198-207] [211-220] nt  (ATTTGGTGTT..AATACCTAAT)
IR2:[339-3444] [350-355] nt  (AAAAAA..TTTTTT)
IR3: [413-422] [425-434] nt  (GTATTCATGC..GCATGAATAC)
Location of nic site [301-302] nt
Conserved sequence flanking the
  nic site 		GGTGT|ATAGC
Note predicted by the oriTfinder
L:198-207;R:211-220;L:339-344;R:350-355;L:413-422;R:425-434;D:301-302
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 687
Name TraI_pG6809-2 insolico
GenBank accession number YP_009328667
Family MOBF
Length 1078 aa
UniProt ID A0A0P0KT65
PDB ID _
Pfam TrwC [PF08751.10], Evalue: 6.70E-80, Aligned region: 12..288
AAA_30 [PF13604.5], Evalue: 4.30E-42, Aligned region: 477..663
Viral_helicase1 [PF01443.17], Evalue: 5.00E-07, Aligned region: 774..899
UvrD_C_2 [PF13538.5], Evalue: 1.10E-08, Aligned region: 851..897
Note putative relaxase
Protein sequence [Download] MLDITTITRQNVTSVVGYYSDAKDDYYSKDSSFTSWQGTGAEALGLSGDVESARFKELLV
GEIDTFTHMQRHVGDAKKERLGYDLTFSAPKGVSMQALIHGDKTIIEAHEKAVAAAVREA
EKLAQARTTRQGKSVTQNTNNLVVATFRHETSRALDPDLHTHAFVMNMTQREDGQWRALK
NDELMRNKMHLGDVYKQELALELTKAGYELRYNSKNNTFDMAHFSDEQIRAFSRRSEQIE
KGLAAMGLTRETADAQTKSRVSMATREKKTEHSREEIHQEWASRAKTLGIDFDNREWQGH
GKPLEADIARNMAPDFTSPEVKADRAIQFAVKSLSERDASFERQKLIQIANKQVLGHATI
ADVEKAYLKAVQKGAIIEGEARYQSTLKVGASVMAETLTRKEWIDSLTNSGMRADKARFA
VDDGIKNGRLKKTSHRVTTVEGIRLERSILTIESRGRGQMPRQLTAEIAGQLLAGKTLKK
EQMRAVTEIVTSKDRFVAAHGYAGTGKSYMTMAAKELLESQGLKVTALAPYGTQKKALED
DGLPARTVAAFLKAKDKKLDEKSVVFIDEAGVIPARQMKQLMEVIEKHNARAVFLGDTSQ
TKAVEAGKPFEQLIKAGMQTSYMKDIQRQKNEVLLEAVKYAAEGNAARALKNITGVNELK
EEAPRLAQLADRYLSLSSEQQDATLIISGTNASRKTLNDYIRGNLGLAGTGETFTLLDRV
DSTQAERRDSRYFSKGQIIIPEQDYKNGMKRGESYQVLDTGPGNKLTVESSSGEQIAFSP
RTHTKLSVYQAVSAELAPGDKVMVTRNDKTLDVANGDRFTVKTVEGEKLTLEDKKGRTVE
LDKKQASYLSYAYATTVHKSQGLTCDRVLFNIDTKSLTTSKDVFYVGISRARHEVEIFTD
DKKSLASSVSRDSPKTTAAEIDRFFGLEARFKDIGRDTSLETRSAEKGLPEATGESMAFN
QKPDEHNMTTGTDYQPVSNAEDAFHLKQNPMDDSVGLRRHEAQQNDAELAHDYAAADDQQ
WSAQEYADYEHYAEASDYDFDSSIYDDYAMPQTSQAEQSHTGKEHTHEHEHEEGGHEI
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1075
Plasmid name pG6809-2
GenBank accession number NC_032101.1
Incompatibility group IncN
Genome size 58120 bp
Coordinate of oriT  [Strand] 44472..45129 [+]
Drug resistance _
Heavy-metal resistance insolico mercury resistance
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Enterobacter cloacae G6809 [550]
Reference
 N/A

Microbial Bioinformatics Group in MML, SJTU