oriTDB

The information of the oriT region

oriTDB accession number	100610
Name	oriT_pRJ9
Sequence Completeness	intact
oriT length	239 nt
oriT sequence	AGTGGCTAGTAATTAGCCACTATTTTTTCGCCAGAAAAAACCCTAAGGGGATTGGGGAAT ATCCCCAACAAGCAGGCGTAGCTAACACGCTAGTGGCTAGCGAAGCCAATGCTTGCCAAA CCACTTTAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGAAGT TATCTTTCGTAGAAAGCGTGGTGGGATATATGAGCGAACAAAACAGATTTGTGGCTAGC
IR (inverted repeat)	IR1: [52-58] [62-68] nt (TTGGGGA..TCCCCAA) IR2: [94-98] [104-108] nt (TGGCT..AGCCA)
Location of nic site	[115-116] nt
Conserved sequence flanking the nic site	TGCTTG\|CCA
Note	predicted by the oriTfinder
	L:52-58;R:62-68;L:94-98;R:104-108;D:115-116

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

AGTGGCTAGTAATTAGCCACTATTTTTTCGCCAGAAAAAACCCTAAGGGGATTGGGGAAT

61

ATCCCCAACAAGCAGGCGTAGCTAACACGCTAGTGGCTAGCGAAGCCAATGCTTGCCAAA

121

CCACTTTAACGACTTGATTAAATTTACTTTATCCTATAAAGTAAATTTATATTAAGAAGT

181

TATCTTTCGTAGAAAGCGTGGTGGGATATATGAGCGAACAAAACAGATTTGTGGCTAGC

Reference
N/A

I. Information of Relaxase

ID	682
Name	MobA_pRJ9
GenBank accession number	YP_009072005
Family	MOB_P
Length	335 aa
UniProt ID	Q8GPJ1
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 9.70E-78, Aligned region: 8..242
Note	putative relaxase
Protein sequence [Download]	MATTKLSATKSTSRAINYAEKRAVEKSGLNCDVDYAKSSFKASRELYGKTDGNQGHVIIQ SFKPDEVTPEQCNQLGLELAEKFAAHHQVAVYTHNDTNHVHNHIVINSINLETGKKFNNN KQALRDLRDFNDQVCRNHDLSVPEKDTARLRYTQTEKAIADPNIKSTAQYSWKDEIREAI DQSQATNIDELKTHLSQYGIEIERVTSKSITYRHLAEDKKVRGRRLGENYNKGGIEDGFE RQIQRRQQERESEHDYQPKRRISNRDESTERDTGLTQPDWDRFAKDTNELERSRKAAESA RFINEKARRDREARIQAERETRTIINNDRGHDYEL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1070
Plasmid name	pRJ9
GenBank accession number	NC_025194.1
Genome size	10406 bp
Coordinate of oriT [Strand]	11..249 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Staphylococcus aureus [1280]

Reference
N/A