 oriTDB accession number | 100601 |
| Name | oriT_Colicin E1  |
Sequence Completeness  | intact |
| oriT length | 83 nt |
| oriT sequence | GGGTGTCGGGGCGCAGCCCTGACCCAGTCACGTAGCGATAGCGGAGTGTATACTGGCTTA ACCATGCGGCATCAGTGCGGATT |
| IR (inverted repeat) | [3-12] [16-25] nt (GTGTCGGGGC..GCCCTGACCC) |
| Location of nic site | [56-57] nt |
| Conserved sequence flanking the nic site | CTGG|CTTA |
| Note | predicted by the oriTfinder |
Visualization of oriT structure Reference| ID | 673 |
| Name | TraI_Colicin E1 |
| GenBank accession number | YP_009070583 |
| Family | MOBF |
| Length | 1763 aa |
| UniProt ID | A0A075M947 |
| PDB ID | _ |
| Pfam | TrwC [PF08751.10], Evalue: 2.20E-74, Aligned region: 17..291 TraI [PF07057.10], Evalue: 7.70E-56, Aligned region: 1441..1563 AAA_30 [PF13604.5], Evalue: 1.70E-34, Aligned region: 975..1167 AAA_19 [PF13245.5], Evalue: 9.80E-09, Aligned region: 980..1115 |
| Note | putative relaxase |
| Protein sequence [Download] | MSKGYTFMMSIAQVRSAGSAGNYYTDKDNYYVLGSMGERWAGRGAEQLGLQGSVDKDVFT RLLEGRLPDGADLSRMQDGSNRHRPGYDLTFSAPKSVSMMAMLGGDKRLIDAHNQAVDFA VRQVEALASTRVMTDGQSETVLTGNLVMALFNHDTSRDQEPQLHTHAVVANVTQHNGEWK TLSSDKVGKTGFIENVYANQIAFGRLYREKLKEQVEALGYETEVVGKHGMWEMPGVPVEA FSGRSQTIREAVGEDASLKSRDVAALDTRKSKQHVDPEIKMVEWMQTLKETGFDIRAYRD AADQRADLRTLTPGPASQDGPDVQQAVTQAIAGLSERKVQFTYTDVLARTVGILPPENGV IERARAGIDEAISREQLIPLDREKGLFTSGIHVLDELSVRALSRDIMKQNRVTVHPEKSV PRTAGYSDAVSVLAQDRPSLAIVSGQGGAAGQRERVAELVMMAREQGREVQIIAADRRSQ MNMKQDERLSGELITGRRQLLEGMTFTPGSTVIVDQGEKLSLKETLTLLDGAARHNVQVL IIDSGQRTGTGSALMAMKDAGVNTYRWQGGEQRPATIISEPDRNVRYARLAGDFAVSVKA GEESVAQVSGVREQAMLTQTIRSELKTQGVLGHPEVTMTALSPVWLDSRSRYLRDMYRPG MVMEQWNPETRSHDRYVIDRVTAQSHSLTLRDAQGETQVVRISSLDSSWSLFRPEKMPVA DGERLRVTGKIPGLRVSGGDRLQVSSVSEDAMTVVVPGRAEPATLPVSDSPFTALKLENG WVETPGHSVSDSATVFASVTQMAMDNATLNGLARSGRDVRLYSSLDETRTAEKLARHPSF TVVSEQIKTRAGETSLETAISHQKSALHTPAQQAIHLALPVVESKKLAFSMVDLLTEAKS FAAEGTGFTELGGEINAQIKRGDLLYVDVAKGYGTGLLVSRASYEAEKSILRHILEGKEA VMPLMERVPGELMEKLTSGQRAATRMILETSDRFTVVQGYAGVGKTTQFRAVMSAVNMLP ESERPRVVGLGPTHRAVGEMRSAGVDAQTLASFLHDTQLQQRSGETPDFSNTLFLLDESS MVGNTDMARAYALIAAGGGRAVASGDTDQLQAIAPGQPFRLQQTRSAADVAIMKEIVRQT PELREAVYSLINRDVERALSGLESVKPSQVPRQEGAWAPEHSVTEFSHSQEAKLAEAQQK AMLKGEAFPDVPMTLYEAIVRDYTGRTPEAREQTLIVTHLNEDRRVLNSMIHDVREKAGE LGKEQVMVPVLNTANIRDGELRRLSTWETHRDALVLVDNVYHRIAGISKDDGLITLQDAE GNTRLISPREAVAEGVTLYTPDTIRVGTGDRMRFTKSDRERGYVANSVWTVTAVSGDSVT LSDGQQTREIRPGQEQAEQHIDLAYAITAHGAQGASETFAIALEGTEGNRKLMAGFESAY VALSRMKQHVQVYTDNRQGWTDAINNAVQKGTAHDVFEPKPDREVMNAERLFSTARELRD VAAGRAVLRQAGLAGGDSPARFIAPGRKYPQPYVALPAFDRNGKSAGIWLNPLTTDDGNG LRGFSGEGRVKGSGDAQFVALQGSRNGESLLADNMQDGVRIARDNPDSGVVVRIAGEGRP WNPGAITGGRVWGDIPDNSVQPGAGNGEPVTAEVLAQRQAEEAIRRETERRADEIVRKMA ENKPDLPDGKTEQAVREIAGQERDRAAITEREAALPEGVLREPQRVREAVREIARENLLQ ERLQQMERDMVRDLQKEKTLGGD |
Reference| ID | 1061 |
| Plasmid name | Colicin E1 |
| GenBank accession number | NC_025157.1 |
| Incompatibility group | ColRNAI |
| Genome size | 1683 bp |
| Coordinate of oriT [Strand] | 93..175 [+] |
| Drug resistance | _ |
| Heavy-metal resistance | _ |
| Virulence factor | _ |
| Xenobiotic degradation | _ |
| Host bacterium [NCBI Taxonomy ID] | Escherichia coli [562] |
Reference| [1] Panayotatos N et al (1981) Cruciform structures in supercoiled DNA. Nature. 289(5797):466-70. [PMID:7464915] |
| [2] Oka A et al (1979) Nucleotide sequence of small ColE1 derivatives: structure of the regions essential for autonomous replication and colicin E1 immunity. Mol Gen Genet. 172(2):151-9. [PMID:384144] |