oriTDB
The information of the oriT region
oriTDB accession number 100599
Name oriT_pCTXM5-1358  insolico
Sequence Completeness intact
oriT length 175 nt
oriT sequence GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAAGGTGG CACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)[2-11] [15-24] nt  (GTTTCGGGGC..GCCCTGAACC)
Location of nic site [55-56] nt
Conserved sequence flanking the
  nic site 		CTGG|CTTA
Note predicted by the oriTfinder
L:2-11;R:15-24;D:55-56
Visualization of oriT structure
Reference
 N/A
I. Information of Relaxase
ID 671
Name MobA_pCTXM5-1358 insolico
GenBank accession number YP_009069712
Family MOBP
Length 515 aa
UniProt ID I6ZWA6
PDB ID _
Pfam Relaxase [PF03432.13], Evalue: 7.20E-15, Aligned region: 66..264
Note putative relaxase
Protein sequence [Download] MPDLSGCGTPFLRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVREL
IDASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRL
ELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGL
PKTTQEAAEAITRGLLSLASSGELKSRQDVTDTLEGAGFEVVRTTKSSISIADPDGGRNI
RLKGAIYEQSFYGLEGLRGEIESAAAEYRRDAESRIQRARAVCQSGTERKREENQRRHQR
PRAEYERGDGEKPAERAADGREDMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAG
RYPGENDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAENDRAGKAV
AERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERDAERARVGLEHAGAEFKRAAAPL
VRELNAIELRQRENEYQKTLELERQPQKTYRGPTL
Reference
 N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
 N/A
 N/A
Information of plasmid
ID 1059
Plasmid name pCTXM5-1358
GenBank accession number NC_025149.1
Genome size 8216 bp
Coordinate of oriT  [Strand] 1959..2133 [+]
Drug resistance _
Heavy-metal resistance _
Virulence factor _
Xenobiotic degradation _
Host bacterium [NCBI Taxonomy ID] Salmonella enterica subsp. enterica serovar Typhimurium [90371]
Reference
[1] Kozyreva VK et al (2014) Long-term dissemination of CTX-M-5-producing hypermutable Salmonella enterica serovar typhimurium sequence type 328 strains in Russia, Belarus, and Kazakhstan. Antimicrob Agents Chemother. 58(9):5202-10. [PMID:24957829]

Microbial Bioinformatics Group in MML, SJTU