oriTDB

The information of the oriT region

oriTDB accession number	100598
Name	oriT_pCTXM5-891
Sequence Completeness	intact
oriT length	175 nt
oriT sequence	GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAAGGTGG CACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC
IR (inverted repeat)	[2-11] [15-24] nt (GTTTCGGGGC..GCCCTGAACC)
Location of nic site	[55-56] nt
Conserved sequence flanking the nic site	CTGG\|CTTA
Note	predicted by the oriTfinder
	L:2-11;R:15-24;D:55-56

Visualization of oriT structure

1...5....10....15....20....25....30....35....40....45....50....55....60

1

GGTTTCGGGGCGCAGCCCTGAACCAGTCACGTAGCGCTAGCGGAGTGTATACTGGCTTAC

61

TATGTTGGCATGGATGCGGAAATCAGTGAAGTGCTTCATGTGGAAGGAGAAAAAAGGTGG

121

CACCGGCGCGTCAGCAGAATATGCGATACAGGATATATTCCGCTTCCTCGCTCAC

Reference
N/A

I. Information of Relaxase

ID	670
Name	MobA_pCTXM5-891
GenBank accession number	YP_009069699
Family	MOB_P
Length	515 aa
UniProt ID	I6ZWA6
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 7.20E-15, Aligned region: 66..264
Note	putative relaxase
Protein sequence [Download]	MPDLSGCGTPFLRKERTMIVKFHPRGRGGGAGPVDYLLGKDRQREGATVLQGKPEEVREL IDASPYVKKYTSGVLSFAEADLPPGQREKLMASFERVLMPGLDKDQYSILWVEHADKGRL ELNFLIPNTELLTGRRLQPYYDRADRPRIDAWQTIVNGRLGLHDPNAPENRRALVTPSGL PKTTQEAAEAITRGLLSLASSGELKSRQDVTDTLEGAGFEVVRTTKSSISIADPDGGRNI RLKGAIYEQSFYGLEGLRGEIESAAAEYRRDAESRIQRARAVCQSGTERKREENQRRHQR PRAEYERGDGEKPAERAADGREDMAHHPDGVSAADRHEREHSVVAGAADNRQLHASSSAG RYPGENDRPDVGSALSGEQRRPEVSYPAAGDANRSHPLRRDDMDPPETGVAENDRAGKAV AERIRAATAGLLAKAGRMGERLRGMAEHVWAYATGERDAERARVGLEHAGAEFKRAAAPL VRELNAIELRQRENEYQKTLELERQPQKTYRGPTL

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1058
Plasmid name	pCTXM5-891
GenBank accession number	NC_025148.1
Genome size	12391 bp
Coordinate of oriT [Strand]	1959..2133 [+]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Salmonella enterica subsp. enterica serovar Typhimurium [90371]

Reference

[1] Kozyreva VK et al (2014) Long-term dissemination of CTX-M-5-producing hypermutable Salmonella enterica serovar typhimurium sequence type 328 strains in Russia, Belarus, and Kazakhstan. Antimicrob Agents Chemother. 58(9):5202-10. [PMID:24957829]