oriTDB

oriT
Relaxosome
T4CP
Plasmid&Host

The information of the oriT region

oriTDB accession number	100597
Name	oriT_pL2-87
Sequence Completeness	intact
oriT length	86 nt
oriT sequence	CGGGGTGTCGGGGCTAAGCCCTGACCAGGTGGTAATCGTATAGCCGTGCGTGCGCGGTTA TACGATTACACATCCTGTCCCGTTTT
IR (inverted repeat)	IR1: [7-15] [17-25] nt (GTCGGGGCT..AGCCCTGAC) IR2: [32-49] [52-69] nt (GTAATCGTATAGCCGTGC..GCGCGGTTATACGATTAC)
Location of nic site	[77-78] nt
Conserved sequence flanking the nic site	CATCCTG\|T
Note	predicted by the oriTfinder
	L:7-15;R:17-25;L:32-49;R:52-69;D:77-78

Visualization of oriT structure

Reference
N/A

I. Information of Relaxase

ID	669
Name	NikB_pL2-87
GenBank accession number	YP_009069160
Family	MOB_P
Length	906 aa
UniProt ID	A0A085NWH8
PDB ID	_
Pfam	Relaxase [PF03432.13], Evalue: 5.00E-51, Aligned region: 61..315
Note	putative relaxase
Protein sequence [Download]	MNAIIPKKRRDGKSSFEDLIAYTSVRDDVPEDELRPDTEVKGEVPHRNRFRRLVDYATRL RNEKFVSLIDVMKDGSQWVNFYGVTCFHNCLSLESAAEEMQKAADLAHFSKDDTDPVFHY ILSWPAHESPRSEQLFDCVRHTLKSLELSKHQYVAAVHTDTDNLHVHVAVNRVHPETGYI NCLPWSQEKLSRACRELELKHGFAPDNGCFVHAPGNRIVRKTALVRERRNAWRRGKKQTF REYIAQMSIAGLREEPAQDWLSLHKRLASDGLYITMQEGELVVKDGWDRAREGVALSSFG PSWTAEKLGRKLGEYQPVPTDIFSQVGTPGRYDPEAINVDIRPEKVAETESLKQYACRHF AERLPAMARNGELKSCLAVHRTLAEAGLWMGIQHGHLVLHDGFDKQQTPVRADSVWPLMT LDYMQDLDGGWQPVPKDIFTQVVPGERFRGHSIGSQPVSDYEWYRMRMGTGPQGAIKREL FSDKESLWGYALIQCRSQIEEMIAGGDFSWQACHEMFARKGLMLQKQHHGLVIVDAFNHE LTPVKASSIHPDLTLSRAEPQAGPFEIAAADIFERVKPECRYNPELAASDEVEPGFRRDP EIRRERREARAAAREDLRARYLAWKEHWRKPDLRYGERLREIHAACRRRKAYIRVQFRDP QLRKLHYHIAEVQRMQALIRLKESVKEERLSLIAEGKWYPLSYRQWVEQQAAQGDRAAVS QLRGWDYRDRRSRNKDKRRTTNADRCVVLCEPGGTPLFNNVAKLEARLQKNGSVHFRDTR TGKNVCTDYGDRVVFYHHTDRNELAEKLDLIAPVLFSRNGKLGFEPEGSYQQFNDVFAEM VAWHNAAGITGNGHFTITRPDVDLHRQRSEQYYREYIRQQTRLSESHDDNYTLRQEKTWE PPSPGM

Reference
N/A

II. Auxiliary DNA-binding protein(s) of the relaxosome
N/A

N/A

Information of plasmid

ID	1057
Plasmid name	pL2-87
GenBank accession number	NC_025145.1
Incompatibility group	IncB/O/K/Z
Genome size	87042 bp
Coordinate of oriT [Strand]	8019..8104 [-]
Drug resistance	_
Heavy-metal resistance	_
Virulence factor	_
Xenobiotic degradation	_
Host bacterium [NCBI Taxonomy ID]	Escherichia coli Lamb-2 [562]

Reference

[1] Wang J et al (2014) Nucleotide sequences of 16 transmissible plasmids identified in nine multidrug-resistant Escherichia coli isolates expressing an ESBL phenotype isolated from food-producing animals and healthy humans. J Antimicrob Chemother. 69(10):2658-68. [PMID:24920651]